Richard S Schwartz

US Patent:

56703511, Sep 23, 1997

Filed:

Dec 30, 1994

Appl. No.:

8/366493

Inventors:

Stephen G. Emerson - Ann Arbor MI
Michael F. Clarke - Ann Arbor MI
Bernhard O. Palsson - Ann Arbor MI
Richard M. Schwartz - Ann Arbor MI

Assignee:

The Regents of the University of Michigan - Ann Arbor MI

International Classification:

C12N 1500
C12N 500

US Classification:

4351723

Abstract:

Methods, including culture media conditions, which provide for ex vivo human stem cell division and stable genetic transformation and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.

US Patent:

57632669, Jun 9, 1998

Filed:

Nov 7, 1994

Appl. No.:

8/290773

Inventors:

Bernhard O. Palsson - Ann Arbor MI
Stephen G. Emerson - Wayne PA
Richard M. Schwartz - San Mateo CA

Assignee:

The Regents of the University of Michigan - Ann Arbor MI

International Classification:

C12M 300

US Classification:

4352891

Abstract:

Methods, compositions and devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The stromal cells and hematopoietic cells may be maintained separately, and both the adherent and non-adherent cells harvested.

US Patent:

54590698, Oct 17, 1995

Filed:

Jan 6, 1994

Appl. No.:

8/178433

Inventors:

Bernhard O. Palsson - Ann Arbor MI
Stephen G. Emerson - Ann Arbor MI
Richard M. Schwartz - Ann Arbor MI

Assignee:

The Regents of the University of Michigan - Ann Arbor MI

International Classification:

C12M 300
C12M 304

US Classification:

4352891

Abstract:

Devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The stromal cells and hematopoietic cells are maintained separately and both the adherent and non-adherent cells are harvested.

US Patent:

53994930, Mar 21, 1995

Filed:

Aug 5, 1991

Appl. No.:

7/740590

Inventors:

Stephen G. Emerson - Ann Arbor MI
Michael F. Clarke - Ann Arbor MI
Bernhard O. Palsson - Ann Arbor MI
Richard M. Schwartz - Ann Arbor MI

Assignee:

The Regents of the University of Michigan - Ann Arbor MI

International Classification:

C12N 1500

US Classification:

4351723

Abstract:

Methods, including culture media conditions, which provide for ex vivo human stem cell division and stable genetic transformation and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.

US Patent:

58888071, Mar 30, 1999

Filed:

Jun 7, 1995

Appl. No.:

8/486247

Inventors:

Bernhard O. Palsson - Ann Arbor MI
Stephen G. Emerson - Ann Arbor MI
Richard M. Schwartz - Ann Arbor MI

Assignee:

The Regents of the University of Michigan - Ann Arbor MI

International Classification:

C12M 306

US Classification:

4352932

Abstract:

Methods, compositions and devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The invention also allows for the separate maintenance of stromal and hematopoietic cells, and to allow for harvesting of both the adherent and non-adherent cells.