Robert H Adolfsen

US Patent:

6348354, Feb 19, 2002

Filed:

Jul 6, 1998

Appl. No.:

09/111162

Inventors:

Robert Adolfsen - Montrose NY
Paul Gherson - Yorktown Heights NY
David Lightbody - Cresskill NJ

Assignee:

Bayer Corporation - Tarrytown NY

International Classification:

G01N 3508

US Classification:

436 53, 436 52, 436 54, 436180, 422 63, 422 81, 422103, 422105, 422108

Abstract:

A method and apparatus for controlling a stream of liquid and air segments wherein the liquid and air segments are selectively aspirated into a first fluid conduit in a plurality of cycles, each cycle beginning with the aspiration of a first air segment and ending with the aspiration of a final air segment. The liquid and air segments are then transferred from the first fluid conduit to a second fluid conduit. The volume of the final air segment of each cycle is then adjusted after the final air segment has moved into the second fluid conduit. Next, the liquid segments and the air segments of each cycle are transferred from the second fluid conduit to a third fluid conduit. The volume of the first air segment of each cycle is then adjusted after the first air segment has moved into the third fluid conduit.

US Patent:

6485980, Nov 26, 2002

Filed:

Apr 3, 2000

Appl. No.:

09/541664

Inventors:

Robert H. Adolfsen - Montrose NY
Paul Gherson - Yorktown Heights NY
David Lightbody - Cresskill NJ

Assignee:

Bayer Corporation - Tarrytown NY

International Classification:

G01N 3565

US Classification:

436 53, 436 52, 436 54, 436180, 422 63, 422 81, 422 681, 422103, 422105, 422108

Abstract:

A method and apparatus for controlling a stream of liquid and air segments wherein the liquid and air segments are selectively aspirated into a first fluid conduit in a plurality of cycles, each cycle beginning with the aspiration of a first air segment and ending with the aspiration of a final air segment. The liquid and air segments are then transferred from the first fluid conduit to a second fluid conduit. The volume of the final air segment of each cycle is then adjusted after the final air segment has moved into the second fluid conduit. Next, the liquid segments and the air segments of each cycle are transferred from the second fluid conduit to a third fluid conduit. The volume of the first air segment of each cycle is then adjusted after the first air segment has moved into the third fluid conduit.

US Patent:

6623971, Sep 23, 2003

Filed:

Jan 11, 1999

Appl. No.:

09/228144

Inventors:

Robert Adolfsen - Montrose NY

Assignee:

Bayer Corporation

International Classification:

G01N 3508

US Classification:

436 53, 436 43, 436180, 436172, 422 681, 422 82, 422 8208, 422 8205, 422105

Abstract:

An apparatus and a method for analyzing a plurality of samples according to an analysis protocol is described, wherein each of the samples is contained within a liquid test package comprising a plurality of liquid and air segments including a reagent segment and wherein during at least a portion of the protocol the reagent segment emits photons. At least one of the liquid test pack includes a buffer segment separated from the reagent segment by a vanish bubble. The apparatus comprises a fluid conduit through which the liquid test packages are flowed, a first luminometer positioned along the fluid conduit for detecting photons emitted by the reagent segment of the at least one of the liquid test packages, a second luminometer positioned along the fluid conduit for detecting photons emitted by the reagent segments of the liquid test packages other than the at least one of the liquid test packages, and a vanish zone contained within the fluid conduit between the first and second luminometers. The reagent segment and the buffer segment of the at least one of the liquid test packages are mixed together in the vanish zone such that the light/photon producing reaction is substantially ceased. The method comprises the steps of inserting the liquid test packages into a fluid conduit, flowing the liquid test packages past a first luminometer positioned along the fluid conduit and detecting photons emitted by the reagent segment of the at least one of the liquid test packages, mixing the reagent segment and the buffer segment of the at least one of the liquid test packages after the photons have been detected, and flowing the liquid test packages past a second luminometer positioned along the fluid conduit and detecting photons emitted by the reagent segments of the liquid test packages other than the at least one of the liquid test packages.

US Patent:

6872571, Mar 29, 2005

Filed:

Apr 3, 2000

Appl. No.:

09/541663

Inventors:

Robert Adolfsen - Montrose NY, US
Paul Gherson - Yorktown Heights NY, US
David Lightbody - Cresskill NJ, US

Assignee:

Bayer Corporation - Tarrytown NY

International Classification:

G01N035/08
G01N001/10
G01N021/00
B01N011/00
G05B001/00

US Classification:

436 53, 436 52, 436 54, 436180, 422 63, 422 81, 422103, 422105, 422108

Abstract:

A method and apparatus for controlling a stream of liquid and air segments wherein the liquid and air segments are selectively aspirated into a first fluid conduit in a plurality of cycles, each cycle beginning with the aspiration of a first air segment and ending with the aspiration of a final air segment. The liquid and air segments are then transferred from the first fluid conduit to a second fluid conduit. The volume of the final air segment of each cycle is then adjusted after the final air segment has moved into the second fluid conduit. Next, the liquid segments and the air segments of each cycle are transferred from the second fluid conduit to a third fluid conduit. The volume of the first air segment of each cycle is then adjusted after the first air segment has moved into the third fluid conduit.

US Patent:

20100112567, May 6, 2010

Filed:

Nov 1, 2007

Appl. No.:

12/447932

Inventors:

Robert Adolfsen - Montrose NY, US
Nicolae Dumitrescu - Stamford CT, US
Michael Avdenko - Rochester NY, US
Dario Svenjak - Yorktown Heights NY, US

Assignee:

Siemens Healthcare Diagnostics Inc. - Tarrytown NY

International Classification:

C12Q 1/68
C12M 1/34

US Classification:

435 6, 4352872

Abstract:

A random access, high-throughput system and method for preparing a biological sample for polymerase chain reaction (PCR) testing are disclosed. The system includes a nucleic acid isolation/purification apparatus and a PCR apparatus. The nucleic acid isolation/purification apparatus magnetically captures nucleic acid (NA) solids from the biological sample and then suspends the NA in elution buffer solution. The PCR testing apparatus provides multiple cycles of the denaturing, annealing, and elongating thermal cycles. More particularly, the PCR testing apparatus includes a multi-vessel thermal cycler array that has a plurality of single-vessel thermal cyclers that is each individually-thermally-controllable so that adjacent single-vessel thermal cyclers can be heated or cooled to different temperatures corresponding to the different thermal cycles of the respective PCR testing process.

US Patent:

48392766, Jun 13, 1989

Filed:

Dec 5, 1984

Appl. No.:

6/678531

Inventors:

Robert H. Adolfsen - Montrose NY
Eddie Hedaya - Hartsdale NY
C. Lam Mak - Valley Cottage NY
Moshe Schwarzberg - Hastings-on-Hudson NY

Assignee:

Technicon Instruments Corporation - Tarrytown NY

International Classification:

G01N 3353
G01N 33543
G01N 33554

US Classification:

435 7

Abstract:

A method and reagent composition provide for the removal or blocking of serum interferences in liposome immunoassays. A carrier or liposome exhibiting at least one domain of phosphoryl ester groupings different from those of the marker liposome is introduced into the reaction mixture, whereby endogenous interfering species competitively bind to such carrier or liposome rather than to the marker liposome. Also, soluble phosphoryl ester groupings can be introduced into the reaction mixture which react with such endogenous species and further reduce serum interferences.

US Patent:

56311672, May 20, 1997

Filed:

Jul 31, 1995

Appl. No.:

8/509692

Inventors:

Robert F. Adolfsen - Montrose NY
Hashem Akhavan-Tafti - Sterling Heights MI
Renuka De Silva - Northville MI
A. Paul Schaap - Grosse Pointe Park MI

Assignee:

Bayer Corporation - Tarrytown NY
Board of Governors Wayne State Univ. - Detroit MI

International Classification:

G01N 3508
G01N 2176

US Classification:

436 53

Abstract:

Improved assay methods utilizing stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Assays are performed by a capsule chemistry analytical assay method wherein fluid capsules comprising discrete aqueous segments containing either a chemiluminescent dioxetane reagent or an activating agent separated by an oil-based isolating fluid are flowed through a conduit, and subsequently reacted to produce light. The improvement comprises using a dioxetane substituted with two or more water-solubilizing groups disposed on the dioxetane structure to provide the chemiluminescence. Compositions comprising such a dioxetane, a non-polymeric surfactant enhancer and optionally a fluorescer, provide enhanced chemiluminescence and eliminate the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems.