Robert R Canfield

US Patent:

7060802, Jun 13, 2006

Filed:

Sep 18, 2000

Appl. No.:

09/664958

Inventors:

Ilya Trakht - New York NY, US
Robert Canfield - Cold Spring NY, US
Gary Kalantarov - Fort Lee NJ, US
Sergei Rudchenko - Bronx NY, US

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

C07K 16/00

US Classification:

53038885, 435326, 5303917

Abstract:

This invention provides monoclonal antibody-producing hybridomas designated 27. F7 and 27. B1. The invention also provides methods for detecting TIP-2 antigen-bearing cancer cells in a sample, detecting the presence of TIP-2 antigen, optionally on the surface of cancer cells, immunohistochemical screening of a tissue section for the presence of TIP-2 antigen bearing cancer cells, diagnosing cancer in a subject, monitoring progression of cancer wherein the cancer cells are TIP-2 antigen-bearing cells, delivering exogenous material to TIP-2 antigen-bearing cancer cells of a human subject, and treating cancer in a human subject. This invention further provides a kit for detecting the presence of TIP-2 antigen-bearing cancer cells. This invention also provides isolated peptides having the amino acid sequences Lys Leu Leu Gly Gly Gln Ile Gly Leu (SEQ ID No:3) and Ser Leu Leu Gly Cys Arg His Tyr Glu Val (SEQ ID NO:4).

US Patent:

7820400, Oct 26, 2010

Filed:

Jun 13, 2006

Appl. No.:

11/453186

Inventors:

Ilya Trakht - New York NY, US
Robert Canfield - Cold Spring NY, US
Gary Kalantarov - Fort Lee NJ, US
Sergei Rudchenko - Bronx NY, US

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

G01N 33/574

US Classification:

435 723, 435 71

Abstract:

The present invention provides monoclonal antibody-producing hybridomas designated 27. F7 and 27. B1. The invention provides a method of detecting TIP-2 antigen bearing cancer cells in a sample. The invention provides a method of detecting TIP-2 antigen on the surface of cancer cells. The invention provides a method for diagnosing cancer in a subject. The invention provides a method for delivering exogenous material to TIP-2 antigen-bearing cancer cells of a human subject. The invention provides a method for treating cancer in a human subject. The invention provides isolated peptides having the amino acid sequences Lys Leu Leu Gly Gly Gln Ile Gly Leu (SEQ. ID No. ) and Ser Leu Leu Gly Cys Arg His Tyr Glu Val (SEQ. ID No. ). The invention provides a method for immunohistochemical screening of a tissue section for the presence of TIP-2 antigen bearing cancer cells. The invention provides a kit for detecting the presence of TIP-2 antigen-bearing cancer cells.

US Patent:

47925280, Dec 20, 1988

Filed:

Feb 19, 1987

Appl. No.:

7/016213

Inventors:

Robert E. Canfield - New York NY
Paul H. Ehrlich - New York NY
William R. Moyle - Piscataway NJ

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

G01N 33537
G01N 33561
G01N 33577

US Classification:

436515

Abstract:

Mixtures of monoclonal antibodies which contain effective assaying amounts of each of at least two monoclonal antibodies that bind to different antigenic sites on the antigen and are capable under appropriate conditions of binding simultaneously to an antigen are useful in enhanced sensitivity assays for the antigen. By utilizing such mixtures in diagnostic assays for important antigens such as the polypeptide human chorionic gonadotropin enhanced sensitivity can be achieved as compared with assays employing individual monoclonal antibodies.

US Patent:

45145059, Apr 30, 1985

Filed:

May 21, 1982

Appl. No.:

6/380959

Inventors:

Robert E. Canfield - New York NY
Paul H. Ehrlich - New York NY
William R. Moyle - Piscataway NJ

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

G01N 3354
G01N 3374
G01N 3376

US Classification:

436500

Abstract:

Mixtures of monoclonal antibodies which contain effective assaying amounts of each of at least two monoclonal antibodies that bind to different antigenic sites on the antigen and are capable under appropriate conditions of binding simultaneously to an antigen are useful in enhanced sensitivity assays for the antigen. By utilizing such mixtures in diagnostic assays for important antigens such as the polypeptide human chorionic gonadotropin enhanced sensitivity can be achieved as compared with assays employing individual monoclonal antibodies.

US Patent:

48513563, Jul 25, 1989

Filed:

Jul 14, 1987

Appl. No.:

7/072802

Inventors:

Robert E. Canfield - New York NY
Elmo G. Armstrong - Bronx NY
Paul H. Ehrlich - New York NY
Steven Birken - Dumont NJ

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

G01N 3376
G01N 33535
G01N 33543

US Classification:

436510

Abstract:

An immunoassay for detecting and measuring hCG in a sample includes an antibody directed to the carboxy terminal portion of the. beta. subunit of hCG and a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the. beta. subunit of hCG that both antibodies can simultaneously bind to hCG, wherein at least one of the antibodies is delectable when both are bound to hCG. In a presently preferred embodiment, an immunoassay for hCG or hCB. beta. in urine includes a purified, labeled or detectable serum-derived antibody directed to the carboxy-terminal portion of the. beta. subunit of hCG and a matrix-bound monoclonal antibody directed to a locus on the. beta. subunit sufficiently remote from the carboxy-terminal portion that both antibodies can simultaneously bind to hCG or hCG. beta.