Robert Hugh Crouch

US Patent:

41429389, Mar 6, 1979

Filed:

Oct 8, 1976

Appl. No.:

5/730920

Inventors:

William S. Stavropoulos - Carmel IN
Robert D. Crouch - Indianapolis IN

Assignee:

The Dow Chemical Company - Midland MI

International Classification:

C07G 702
G01N 3114

US Classification:

195 63

Abstract:

Triglycerides in biological fluids containing interfering substances, such as phospholipids, are determined by hydrolyzing the triglycerides to glycerol in a short time span at moderate temperatures with a solution of alkali metal hydroxide in methanol, and treating the resultant hydrolysis mixture with a water soluble magnesium salt to precipitate the interfering substances. Glycerol is then colorimetrically determined in the resultant supernatant solution by incubating with a single reagent-substrate composition to develop a measurable color.

US Patent:

40010899, Jan 4, 1977

Filed:

Mar 20, 1974

Appl. No.:

5/452879

Inventors:

William S. Stavropoulos - Carmel IN
Robert D. Crouch - Indianapolis IN

Assignee:

The Dow Chemical Company - Midland MI

International Classification:

C12K 104
G01N 3100

US Classification:

1951035R

Abstract:

A novel reagent and method for colorimetrically determining the concentration of triglycerides in biological fluids, and for rapid determination of glycerol are described. Triglycerides are hydrolyzed in methanolic base to glycerol, and glycerol content is determined colorimetrically.