Robert J Defranco

US Patent:

54627486, Oct 31, 1995

Filed:

Jan 14, 1994

Appl. No.:

8/183104

Inventors:

David H. Lloyd - Daly City CA
Robert J. DeFranco - San Carlos CA
Charles S. Ladd - Union City CA

Assignee:

Applied Biosystems, Inc. - Foster City CA

International Classification:

A61K 910
A61K 916
C07K 106
C07K 110
B65D 326
B01J 1918

US Classification:

424484

Abstract:

A cartridge containing a dry polymer composition carrying trapped biopolymer subunits for use in synthesis of biopolymer chains provides a discrete unit for stepwise supply of subunits, such as protected amino acids, in an automated apparatus for biopolymer synthesis. The composition is swellable in organic solvent to release and supply the subunits to a growing biopolymer chain immobilized on a polymer support. An automated synthesizer uses the cartridges for sequential subunit supply and monitors conductivity of deprotection reagents to control cycling and timing of steps.

US Patent:

52982599, Mar 29, 1994

Filed:

Nov 5, 1991

Appl. No.:

7/788322

Inventors:

David H. Lloyd - San Francisco CA
Robert J. DeFranco - San Carlos CA

Assignee:

Applied Biosystems, Inc. - Foster City CA

International Classification:

A61K 910
A61K 916
C07K 106
B65D 326

US Classification:

424486

Abstract:

A polymer composition having a biopolymer subunit entrapped in a swellable polymer matrix is described. The composition may be supplied in a disposable cartridge, for use in delivering a solution of the subunit in an automated biopolymer synthesis operation.

US Patent:

56656033, Sep 9, 1997

Filed:

Jul 8, 1994

Appl. No.:

8/272496

Inventors:

Victoria L. Boyd - San Carlos CA
MeriLisa Bozzini - Burlingame CA
Robert J. DeFranco - San Carlos CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

A61K 3802

US Classification:

436 90

Abstract:

A method of forming a thiohydantoin from an N-protected amino acid is described. The method employs a phosphate compound selected from the group consisting of (R. sub. 1 O)(R. sub. 2 O)P(. dbd. O)X and (R. sub. 1 O)(R. sub. 2 O)P(. dbd. O)--O--P(. dbd. O)(OR. sub. 3)(OR. sub. 4) to form acylphosphate moieties from the carboxyl groups of internal aspartic acid and glutamic acid residues and an acylphosphate moiety at a C-terminal carboxyl. The later acylphosphate, unlike the internal acylphosphates, spontaneously cyclizes to an oxazolone, which is less reactive with nucleophilic reagents. R. sub. 1 and R. sub. 2 are each alkyl, aryl, or alkaryl groups which are the same or different and which may be covalently linked to each other; R. sub. 3 and R. sub. 4 are each alkyl, aryl, or alkaryl groups which are the same or different and which may be covalently linked to each other; and X is a leaving group, such as chlorine or bromine, which is substantially unreactive towards thiohydantoins. The acylphosphate and oxazolone moieties are then reacted with a thiocyanate reagent under conditions effective to convert the internal acylphosphates to amides and the terminal oxazolone to thiohydantoin, thereby permitting selective C-terminal thiodantionation of aspartic acid- and/or glutamic acid-containing proteins.

US Patent:

39753999, Aug 17, 1976

Filed:

Aug 6, 1974

Appl. No.:

5/495199

Inventors:

Robert Jay DeFranco - Wilmington DE
Richard M. Scribner - Wilmington DE

Assignee:

E. I. Du Pont de Nemours and Company - Wilmington DE

International Classification:

C07D20728
C07D20730

US Classification:

2603262

Abstract:

Certain 1,5-disubstituted 2-pyrrolidinones, and their ring unsaturated analogs, resemble natural prostaglandins. The compounds are easier to prepare than the prostaglandins since they have at least one fewer center of asymmetry and accordingly fewer isomers are produced. Exemplary is 7-[2-oxo-5-(3-hydroxy-1-oct-1-enyl)-1-pyrrolidinyl]heptanoic acid of the formula: ##SPC1##.