Robert D Lowery

US Patent:

6511815, Jan 28, 2003

Filed:

Dec 20, 1999

Appl. No.:

09/467628

Inventors:

Thomas J. Burke - Madison WI
Randall E. Bolger - Oregon WI
Kerry M. Ervin - Middleton WI
Robert G. Lowery - Brooklyn WI
William J. Checovich - Madison WI

Assignee:

PanVera LLC - Madison WI

International Classification:

G01N 3353

US Classification:

435 78, 435 6, 435 691, 435325, 4353201, 530350

Abstract:

The system comprises mixing a fluorescence-emitting compound that binds to the steroid hormone receptors in a solution containing the steroid hormone receptors. Then, measuring the fluorescence polarization of the solution. Subsequently, incubating the solution with at least one molecule that may compete with the compound for interaction with the steroid hormone receptors. Measuring the fluorescence polarization of the solution again. Finally, comparing the fluorescence polarization measurements to quantify any competitive interaction.

US Patent:

6551790, Apr 22, 2003

Filed:

Dec 6, 1999

Appl. No.:

09/455202

Inventors:

Olga Trubetskoy - Middleton WI
Robert G. Lowery - Brooklyn WI

Assignee:

PanVera LLC - Madison WI

International Classification:

C12Q 148

US Classification:

435 15, 435 18, 435968

Abstract:

A fluorescence polarization process used to identify activity of conjugative enzymes involved in xenobiotic transformations, such as glucuronosyltransferases is provided.

US Patent:

7332278, Feb 19, 2008

Filed:

Jan 30, 2004

Appl. No.:

10/769578

Inventors:

Robert G. Lowery - Madison WI, US
John Majer - Madison WI, US

Assignee:

Bellbrook Labs, LLC - Madison WI

International Classification:

C12Q 1/70

US Classification:

435 6

Abstract:

The present invention relates to methods for detecting, quantifying and high throughput screening of donor-products and the catalytic activities generating the donor-products in group-transfer reactions. The invention further provides immunoassays, antibodies and kits that may be used to practice the methods of the invention.

US Patent:

7355010, Apr 8, 2008

Filed:

May 26, 2005

Appl. No.:

11/137947

Inventors:

Robert Lowery - Belleville WI, US
Karen Kleman-Leyer - Madison WI, US
Matt Staeben - Belleville WI, US
Thane Westermeyer - Sun Prairie WI, US

Assignee:

BellBrook Labs, LLC - Madison WI

International Classification:

C07K 16/00
C07K 16/44

US Classification:

5303871, 53038821, 5303889, 5303898

Abstract:

The present invention relates to methods for detecting, quantifying and high throughput screening of donor-products and the catalytic activities generating the donor-products in group-transfer reactions. The invention further provides immunoassays, antibodies and kits that may be used to practice the methods of the invention.

US Patent:

7378505, May 27, 2008

Filed:

Feb 14, 2006

Appl. No.:

11/353500

Inventors:

Robert Lowery - Belleville WI, US
Karen Kleman-Leyer - Madison WI, US
Matt Staeben - Belleville WI, US
Thane Westermeyer - Sun Prairie WI, US

Assignee:

BellBrook Labs LLC - Madison WI

International Classification:

C07K 16/44

US Classification:

5303871, 53038821, 5303891, 5303898

Abstract:

The present invention relates to methods for detecting, quantifying and high throughput screening of donor-products and the catalytic activities generating the donor-products in group-transfer reactions catalyzed by adenosine triphosphatase (ATPase) or guanine triphosphatase (GTPase). The invention further provides immunoassays, antibodies and kits that may be used to practice the methods of the invention.

US Patent:

7847066, Dec 7, 2010

Filed:

Feb 12, 2008

Appl. No.:

12/029932

Inventors:

Robert Lowery - Belleville WI, US
Karen Kleman-Leyer - Madison WI, US
Matt Staeben - Belleville WI, US
Thane Westermeyer - Sun Prairie WI, US

Assignee:

Bellbrook Labs, LLC - Madison WI

International Classification:

C07K 16/00

US Classification:

5303871

Abstract:

The present invention relates to methods for detecting, quantifying and high throughput screening of donor-products and the catalytic activities generating the donor-products in group-transfer reactions. The invention further provides immunoassays, antibodies and kits that may be used to practice the methods of the invention.

US Patent:

8088897, Jan 3, 2012

Filed:

Apr 25, 2008

Appl. No.:

12/109628

Inventors:

Robert Lowery - Belleville WI, US
Karen Kleman-Leyer - Madison WI, US
Matt Staeben - Belleville WI, US
Thane Westermeyer - Sun Prairie WI, US

Assignee:

BellBrook Labs, Inc. - Madison WI

International Classification:

C07K 16/44

US Classification:

5303871, 53038821, 5303891, 5303898

Abstract:

An isolated antibody produced against a donor product of an enzymatically catalyzed group transfer reaction is disclosed. In one aspect, the antibody has the ability to preferentially recognize the donor-product guanosine diphosphate (GDP), in the presence of the donor molecule guanosine triphosphate (GTP). Immunuoassays using compositions including such antibodies and kits for performing the immunoassays are also disclosed.

US Patent:

8546536, Oct 1, 2013

Filed:

Aug 28, 2009

Appl. No.:

13/060895

Inventors:

Robert G. Lowery - Belleville WI, US
Thomas K. Zielinski - Fitchburg WI, US

Assignee:

Bellbrook Labs, LLC - Madison WI

International Classification:

C07K 1/00
C12N 9/00
C12N 9/14

US Classification:

530350, 435183, 435195

Abstract:

The present invention relates to novel engineered Ga proteins and assay methods of using such proteins to advance drug discovery. Engineered Ga proteins described by the invention contain alterations of at least one and preferably two or more amino acid residues that are highly conserved among all four subfamilies of Ga proteins. A preferred engineered protein disclosed here is a double mutant, Gαπ R178M A326S. This specific combination of mutations yields an unexpectedly amplified effect on Ga function both in terms of GTPase activity (GTP hydrolysis) and GDP dissociation. This synergistic effect may have a profound influence on the way GPCR signaling pathways are examined for the development of new pharmacotherapies, particularly in the field of central nervous system disorders such as Parkinson's disease.