Robert D Potter

US Patent:

8541219, Sep 24, 2013

Filed:

Apr 22, 2010

Appl. No.:

12/765253

Inventors:

Robert Potter - San Marcos CA, US
Kim Rosenthal - Laytonsville MD, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12N 9/00
C12N 9/12
C07K 1/00
C07K 14/00
C07K 17/00
C12Q 1/68
C12P 19/34

US Classification:

435183, 435194, 435 61, 435 911, 530350

Abstract:

The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

US Patent:

20060094050, May 4, 2006

Filed:

Nov 3, 2005

Appl. No.:

11/265159

Inventors:

Robert Potter - San Marcos CA, US
Kim Rosenthal - Laytonsville MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12N 9/22

US Classification:

435006000, 435199000

Abstract:

The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

US Patent:

20060292578, Dec 28, 2006

Filed:

Jun 28, 2005

Appl. No.:

11/170762

Inventors:

Weidong Zheng - Carlsbad CA, US
Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US
David Mandelman - Carlsbad CA, US

International Classification:

C12Q 1/68
C07H 21/04
C12P 21/06
C12P 19/34
C12N 9/22
C12N 15/74
C12N 1/21

US Classification:

435006000, 435069100, 435091200, 435199000, 435252300, 435471000, 536023200

Abstract:

A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,

US Patent:

20080254525, Oct 16, 2008

Filed:

Mar 20, 2008

Appl. No.:

12/052682

Inventors:

Weidong Zheng - Carlsbad CA, US
Jun E. Lee - San Diego CA, US
Robert Jason Potter - San Marcos CA, US
David Mandelman - Carlsbad CA, US

Assignee:

INVITROGEN CORPORATION - Carlsbad CA

International Classification:

C12N 9/10

US Classification:

435193

Abstract:

A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,

US Patent:

20220372522, Nov 24, 2022

Filed:

Oct 7, 2020

Appl. No.:

17/766998

Inventors:

- Carlsbad CA, US
Robert POTTER - San Marcos CA, US

International Classification:

C12N 15/90
C12N 9/22
C12N 15/11
C07K 14/245

Abstract:

The present disclosure relates, in part, to improved methods of making single-stranded DNA (ssDNA) from double-stranded DNA (dsDNA), as well as use of the resulting ssDNA for genome engineering. The disclosure also relates, in part, to improved methods of genetic modification using single stranded DNA binding proteins.

US Patent:

20200080077, Mar 12, 2020

Filed:

Aug 9, 2019

Appl. No.:

16/536983

Inventors:

- CARLSBAD CA, US
Robert POTTER - San Marcos CA, US
Namritha RAVINDER - San Diego CA, US
Shantanu KUMAR - Carlsbad CA, US

International Classification:

C12N 15/10
C12N 15/113
C12N 15/90

Abstract:

The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.

US Patent:

20180327738, Nov 15, 2018

Filed:

Nov 18, 2016

Appl. No.:

15/777182

Inventors:

- Carlsbad CA, US
Robert Jason POTTER - San Marcos CA, US
Jason CARTE - Carlsbad CA, US

International Classification:

C12N 15/10
C12N 9/22

Abstract:

The present disclosure generally relates to compositions and methods for genetic modification of cells. In particular, the disclosure relates to stabilized reagents for genome alteration (e.g., site specific nucleases), as well as stabilized reagents for non-genetic code altering modification (e.g., DNA methylation). Stabilization methods include storage of reagents at suitable temperatures, conversion to dry forms, and chemical modifications.

US Patent:

20180023075, Jan 25, 2018

Filed:

May 25, 2017

Appl. No.:

15/605586

Inventors:

- Carlsbad CA, US
Robert POTTER - San Marcos CA, US
Namritha RAVINDER - San Diego CA, US
Shantanu KUMAR - Carlsbad CA, US

International Classification:

C12N 15/10
C12N 15/113

Abstract:

The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.