Robert W Potter

US Patent:

8541219, Sep 24, 2013

Filed:

Apr 22, 2010

Appl. No.:

12/765253

Inventors:

Robert Potter - San Marcos CA, US
Kim Rosenthal - Laytonsville MD, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12N 9/00
C12N 9/12
C07K 1/00
C07K 14/00
C07K 17/00
C12Q 1/68
C12P 19/34

US Classification:

435183, 435194, 435 61, 435 911, 530350

Abstract:

The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

US Patent:

20060094050, May 4, 2006

Filed:

Nov 3, 2005

Appl. No.:

11/265159

Inventors:

Robert Potter - San Marcos CA, US
Kim Rosenthal - Laytonsville MD, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 1/68
C12N 9/22

US Classification:

435006000, 435199000

Abstract:

The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

US Patent:

20060105348, May 18, 2006

Filed:

Nov 15, 2004

Appl. No.:

10/987453

Inventors:

Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US
Jonathan Nery - Escondido CA, US

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435006000, 536025320

Abstract:

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

US Patent:

20060275792, Dec 7, 2006

Filed:

Feb 28, 2006

Appl. No.:

11/365383

Inventors:

Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

Disclosed are methods and materials for enhancing the efficiency, specificity and/or specificity of nucleic acid amplification reactions (e.g., PCR amplification reactions). The disclosed inventions involve the use of nonspecific double-stranded DNA binding proteins in amplification reactions. The methods described herein are useful in a wide variety of molecular biology applications including allele specific PCR.

US Patent:

20060292578, Dec 28, 2006

Filed:

Jun 28, 2005

Appl. No.:

11/170762

Inventors:

Weidong Zheng - Carlsbad CA, US
Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US
David Mandelman - Carlsbad CA, US

International Classification:

C12Q 1/68
C07H 21/04
C12P 21/06
C12P 19/34
C12N 9/22
C12N 15/74
C12N 1/21

US Classification:

435006000, 435069100, 435091200, 435199000, 435252300, 435471000, 536023200

Abstract:

A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,

US Patent:

20070020622, Jan 25, 2007

Filed:

Sep 16, 2002

Appl. No.:

10/244081

Inventors:

Jun Lee - San Diego CA, US
Gary Gerard - Frederick MD, US
Harini Shandilya - Bethesda MD, US
Katherine Griffiths - Hornsby, AU
Moreland Gibbs - Marsfield, AU
Peter Bergquist - Auckland, NZ
Robert Potter - San Marcos CA, US

International Classification:

C12Q 1/68
C07H 21/04
C12N 9/22

US Classification:

435006000, 435069100, 435199000, 435320100, 435325000, 536023200

Abstract:

The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may posses both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.

US Patent:

20070059713, Mar 15, 2007

Filed:

Sep 9, 2005

Appl. No.:

11/222029

Inventors:

Jun Lee - San Diego CA, US
Robert Potter - San Marcos CA, US
David Mandelman - Carlsbad CA, US

International Classification:

C12Q 1/68
C07H 21/04
C12P 21/06
C12N 9/22
C12N 15/74

US Classification:

435006000, 435069100, 435199000, 435252300, 435471000, 536023200

Abstract:

Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).

US Patent:

20080093125, Apr 24, 2008

Filed:

Mar 26, 2007

Appl. No.:

11/691445

Inventors:

Jared Potter - San Carlos CA, US
Robert Potter - Rio Rancho NM, US
James Basler - Redwood City CA, US
Thomas Wideman - Milton MA, US

Assignee:

Potter Drilling, LLC - Redwood City CA

International Classification:

E21B 7/18

US Classification:

175067000, 175091000

Abstract:

System and methods for creating shaped, non-circular boreholes in rocks especially for use with geothermal heat pump applications and for increasing wellbore support in applications such as horizontal oil and gas drilling are described. The systems and methods when applied to geothermal heat pumps create an elliptical shaped hole that is optimized for placing heat transfer tubes with a minimum of grout used. The significantly reduced cross-sectional area of the elliptical borehole also increases the overall drilling rate in rock and especially in hard rocks. In horizontal hard-rock drilling, creation of a horizontal non-circular borehole or modification of a circular borehole to a non-circular geometry is used to stabilize the borehole prior to casing insertion, and may also allow the use of lower mud pressures improving drilling rates. The system uses a non-contacting drilling system which in one embodiment uses a supersonic flame jet drilling system with a movable nozzle that swings between pivot points. In a second embodiment the elliptical shaped hole is created by an abrasive fluid or particle bearing-fluid or air jet drill that moves between pivot points. In another embodiment a non-contacting drill can use dual parallel nutating nozzles that create a pair of overlapping circular holes. The non-circular shaped hole is created by either the high temperature flame or water-particle jet or chemically active fluid jet as it removes rock material by erosion, dissolution and or thermal spalling. Modifications of circular boreholes to a generally elliptical shape can also be done using milling or jetting techniques.