Rohini P Dhulipala

US Patent:

20090143570, Jun 4, 2009

Filed:

Nov 25, 2008

Appl. No.:

12/277420

Inventors:

Miao Jiang - Cedar Knolls NJ, US
Mark S. Briggs - Cardiff, GB
Rohini Dhulipala - Kendall Park NJ, US
Yuyang Christine Cai - Cranbury NJ, US
Renee E. Bruno - Union NJ, US

Assignee:

GE HEALTHCARE BIO-SCIENCES CORP. - PISCATAWAY NJ

International Classification:

C07K 1/16
C07H 21/04
C07H 21/02

US Classification:

530413, 536 231

Abstract:

The invention provides systems, methods and kits for the separation and/or purification of at least two cellular components selected from genomic DNA, RNA and proteins. The method includes first lysing a biological sample to generate an aqueous solution containing the cellular components; then applying the aqueous solution to a first mineral support under conditions for genomic DNA to bind; and collecting the flowthrough which contains unbound total RNA and proteins. The method further includes applying the flowthrough to a second mineral support under conditions for RNA to bind, and collecting the flowthrough which contains proteins. The genomic DNA and total RNA bound can be eluted while the protein in the flowthrough can be further purified. Further the total RNA isolated could be used to isolate small RNA such as microRNA.

US Patent:

20110172405, Jul 14, 2011

Filed:

Sep 17, 2009

Appl. No.:

13/063546

Inventors:

Rohini Dhulipala - Amherst NY, US
Yuyang Christine Cai - Cranbury NJ, US
Miao Jiang - Cedar Knolls NJ, US
Mubasher Dar - North Brunswick NJ, US

Assignee:

GE HEALTHCARE BIO-SCIENCES CORP. - PISCATAWAY NJ

International Classification:

C07H 21/02

US Classification:

536 231

Abstract:

This invention relates to a simple and rapid method for the extraction and purification of small RNA from a sample solution. Accordingly, a sample is first mixed with an organic solvent to form a mixture containing the solvent. The mixture is applied to a first mineral support for large RNA to bind. The filtrate is collected which contain unbound small RNA, and is mixed with a second organic solvent to form a second mixture containing the second solvent. This second mixture is applied to a second mineral support for small RNA to bind. After a wash step, the small RNA is eluted. Also provided is a method for the isolation of large RNA, by eluting the large RNA from the first mineral support. In addition, total protein is present in the filtrate and can be isolated by a conventional method.

US Patent:

20120271042, Oct 25, 2012

Filed:

Jun 28, 2012

Appl. No.:

13/535953

Inventors:

MIAO JIANG - CEDAR KNOLLS NJ, US
MARK S. BRIGGS - CARDIFF, GB
ROHINI DHULIPALA - KENDALL PARK NJ, US
YUYANG CHRISTINE CAI - CRANBURY NJ, US
RENEE E. BRUNO - UNION NJ, US

Assignee:

GE HEALTHCARE BIO-SCIENCES CORP. - PISCATAWAY NJ

International Classification:

C07H 1/08
C07H 21/02
C09K 3/00
C07K 1/14
C07K 1/30
C07K 1/16
C07H 21/04
C07K 14/00

US Classification:

530417, 536 254, 530412, 530418, 252184

Abstract:

The invention provides systems, methods and kits for the separation and/or purification of at least two cellular components selected from genomic DNA, RNA and proteins. The method includes first lysing a biological sample to generate an aqueous solution containing the cellular components; then applying the aqueous solution to a first mineral support under conditions for genomic DNA to bind; and collecting the flowthrough which contains unbound total RNA and proteins. The method further includes applying the flowthrough to a second mineral support under conditions for RNA to bind, and collecting the flowthrough which contains proteins. The genomic DNA and total RNA bound can be eluted while the protein in the flowthrough can be further purified. Further the total RNA isolated could be used to isolate small RNA such as microRNA.

US Patent:

20080003602, Jan 3, 2008

Filed:

Dec 22, 2005

Appl. No.:

11/722089

Inventors:

John Nelson - Clifton Park NY, US
R. Duthie - Schenectady NY, US
Rohini Dhulipala - Kendall Park NJ, US
Gregory Grossmann - Halfmoon NY, US
Anuradha Sekher - Niskayuna NY, US

Assignee:

GE HEALTHCARE BIO-SCIENCES CORP. - PISCATAWAY NJ

International Classification:

C12Q 1/68
C12N 9/00
C12N 9/12
C12P 19/34

US Classification:

435006000, 435183000, 435194000, 435091200

Abstract:

Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.

US Patent:

20180320172, Nov 8, 2018

Filed:

Apr 30, 2018

Appl. No.:

15/966353

Inventors:

- Marlborough MA, US
R. Scott Duthie - Schenectady NY, US
Rohini Dhulipala - Kendall Park NJ, US
Gregory A. Grossman - Halfmoon NY, US
Anuradha Sekher - Belle Mead NJ, US

International Classification:

C12N 15/10
C12Q 1/6865

Abstract:

Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.