B. DUANE PRICE - MADISON WI, US ROLAND R. RUECKERT - MADISON WI, US MICHAEL A. KROL - MADISON WI, US PAUL G. AHLQUIST - MADISON WI, US
International Classification:
C12P019/34 C12N007/00
US Classification:
435/091100, 435/235100
Abstract:
A method of synthesizing particles containing nucleic acid sequences is disclosed. This method comprises the steps of introducing a non-endogenous packageable nucleic acid sequence and a sequence encoding a suitable non-endogenous capsid protein into yeast cells and inducing particle assembly, wherein at least one nucleic acid is encapsidated. Preferably, the particle is an infectious virion and the method additionally comprises the step of inducing viral nucleic acid replication before particle assembly. A method of replicating an animal or Nodaviridae virus nucleic acid sequences in yeast is also disclosed.
Method Of Producing Particles Containing Nucleic Acid Sequences In Yeast
B. Price - Madison WI, US Roland Rueckert - Madison WI, US Michael Krol - Madison WI, US Paul Ahlquist - Madison WI, US
International Classification:
C12P019/34 C12N007/00
US Classification:
435/091200, 435/235100, 435/320100
Abstract:
A method of synthesizing particles containing nucleic acid sequences is disclosed. This method comprises the steps of introducing a non-endogenous packageable nucleic acid sequence and a sequence encoding a suitable non-endogenous capsid protein into yeast cells and inducing particle assembly, wherein at least one nucleic acid is encapsidated. Preferably, the particle is an infectious virion and the method additionally comprises the step of inducing viral nucleic acid replication before particle assembly. A method of replicating an animal or Nodaviridae virus nucleic acid sequences in yeast is also disclosed.
Method Of Producing Particles Containing Nucleic Acid Sequences In Yeast
B. Duane Price - Madison WI Roland R. Rueckert - Madison WI Michael A. Krol - Madison WI Paul G. Ahlquist - Madison WI
Assignee:
Wisconsin Alumni Research Foundation - Madison WI
International Classification:
C12P 2106
US Classification:
435 691
Abstract:
A method of synthesizing particles containing nucleic acid sequences is disclosed. This method comprises the steps of introducing a non-endogenous packageable nucleic acid sequence and a sequence encoding a suitable non-endogenous capsid protein into yeast cells and inducing particle assembly, wherein at least one nucleic acid is encapsidated. Preferably, the particle is an infectious virion and the method additionally comprises the step of inducing viral nucleic acid replication before particle assembly. A method of replicating an animal or Nodaviridae virus nucleic acid sequences in yeast is also disclosed.