Ruiming M Zou

US Patent:

7432298, Oct 7, 2008

Filed:

May 4, 2004

Appl. No.:

10/837983

Inventors:

Joe Y. L. Lam - Castro Valley CA, US
Steven M. Menchen - Fremont CA, US
Ruiming Zou - Foster City CA, US
Scott C. Benson - Alameda CA, US

Assignee:

Applied Biosystems Inc. - Foster City CA

International Classification:

A61K 31/352
C07D 311/88

US Classification:

514459, 549227

Abstract:

Fluorescent polymeric materials are disclosed comprising a polymer and one or more lipid soluble rhodamine dyes. The materials are especially useful in the preparation of multicolored microparticles, especially multicolored polystyrene microparticle, for use in the multiplexed analysis of a plurality of analytes in a single sample. When excited by a light source, the materials give off a unique emission based on the nature, concentration and ratio of the dyes therein. Methods of preparing and using said materials are also disclosed.

US Patent:

7491830, Feb 17, 2009

Filed:

May 4, 2004

Appl. No.:

10/837621

Inventors:

Joe Y. L. Lam - Castro Valley CA, US
Steven M. Menchen - Fremont CA, US
Ruiming Zou - Foster City CA, US
Scott C. Benson - Alameda CA, US

Assignee:

Applied Biosystems Inc. - Foster City CA

International Classification:

C09B 11/24

US Classification:

548418, 549382, 549388, 549394

Abstract:

Fluorescent phenyl xanthene dyes are described that comprise any fluorescein, rhodamine or rhodol comprising a particular C9 phenyl ring. One or both of the ortho groups on the lower C9 phenyl ring is ortho substituted with a group selected from alkyl, heteroalkyl, alkoxy, halo, haloalkyl, amino, mercapto, alkylthio, cyano, isocyano, cyanato, mercaptocyanato, nitroso, nitro, azido, sulfeno, sulfinyl, and sulfino. In one embodiment, halo and/or hydroxy groups are used. Optimal dyes contain a lower C9 phenyl ring in which both ortho groups are the same and the lower ring exhibits some form a symmetry relative to an imaginary axis running from the phenyl rings point of attachment to the remainder of the xanthene dye through a point para to the point of attachment. The phenyl xanthene dyes may be activated. Furthermore, the phenyl xanthene dyes may be conjugated to one or more substances including other dyes.

US Patent:

7985602, Jul 26, 2011

Filed:

Aug 14, 2007

Appl. No.:

11/838793

Inventors:

Ronald J. Graham - San Ramon CA, US
Ruiming Zou - Foster City CA, US
Krishna G. Upadhya - Union City CA, US
Scott C. Benson - Alameda CA, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

G01N 33/53
C07D 221/18

US Classification:

436800, 546 58, 546 61, 546102

Abstract:

The present teachings generally relate to fluorescent dyes, linkable forms of fluorescent dyes, energy transfer dyes, reagents labeled with fluorescent dyes and uses thereof.

US Patent:

8383841, Feb 26, 2013

Filed:

Jan 9, 2009

Appl. No.:

12/351504

Inventors:

Joe Y. L. Lam - Castro Valley CA, US
Steven M. Menchen - Fremont CA, US
Ruiming Zou - Foster City CA, US
Scott C. Benson - Alameda CA, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

C09B 11/06
C09B 11/24

US Classification:

549382, 549384, 549388, 549394

Abstract:

Fluorescent phenyl xanthene dyes are described that comprise any fluorescein, rhodamine or rhodol comprising a particular C9 phenyl ring. One or both of the ortho groups on the lower C9 phenyl ring is ortho substituted with a group selected from alkyl, heteroalkyl, alkoxy, halo, haloalkyl, amino, mercapto, alkylthio, cyano, isocyano, cyanato, mercaptocyanato, nitroso, nitro, azido, sulfeno, sulfinyl, and sulfino. In one embodiment, halo and/or hydroxy groups are used. Optimal dyes contain a lower C9 phenyl ring in which both ortho groups are the same and the lower ring exhibits some form a symmetry relative to an imaginary axis running from the phenyl rings point of attachment to the remainder of the xanthene dye through a point para to the point of attachment. The phenyl xanthene dyes may be activated. Furthermore, the phenyl xanthene dyes may be conjugated to one or more substances including other dyes.

US Patent:

8618161, Dec 31, 2013

Filed:

Feb 14, 2011

Appl. No.:

13/026690

Inventors:

Joe Y. L. Lam - Castro Valley CA, US
Steven M. Menchen - Fremont CA, US
Ruiming Zou - Foster City CA, US
Scott C. Benson - Alameda CA, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

A61K 31/352
C07D 311/92

US Classification:

514453, 549382

Abstract:

Fluorescent polymeric materials are disclosed comprising a polymeric particle and one or more lipid soluble rhodamine dyes, comprising the following core structure:.

US Patent:

20060167257, Jul 27, 2006

Filed:

Sep 16, 2005

Appl. No.:

11/229012

Inventors:

Ronald Graham - San Ramon CA, US
Ruiming Zou - Foster City CA, US
Krishna Upadhya - Union City CA, US
Scott Benson - Alameda CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07D 221/18
C07D 221/22

US Classification:

546042000, 546061000, 546102000

Abstract:

The present teachings generally relate to fluorescent dyes, linkable forms of fluorescent dyes, energy transfer dyes, reagents labeled with fluorescent dyes and uses thereof.

US Patent:

20080071070, Mar 20, 2008

Filed:

Apr 2, 2007

Appl. No.:

11/695548

Inventors:

Scott Benson - Alameda CA, US
Ruiming Zou - Foster City CA, US
Krishna Upadhya - Union City CA, US
Paul Kenney - Sunnyvale CA, US
Jonathan Cassel - Half Moon Bay CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07H 19/06
C07D 491/22
C07H 19/16
C07H 21/00
C07D 493/10

US Classification:

536023100, 536025320, 536026600, 536028510, 546015000, 548407000, 549220000, 549265000

Abstract:

The present disclosure provides reagents that can be used to label synthetic oligonucleotides with rhodamine dyes or dye networks that contain rhodamine dyes.

US Patent:

20090072197, Mar 19, 2009

Filed:

Sep 29, 2008

Appl. No.:

12/240194

Inventors:

Joe Y.L. LAM - Castro Valley CA, US
Steven M. Menchen - Fremont CA, US
Ruiming Zou - Foster City CA, US
Scott C. Benson - Alameda CA, US

Assignee:

Applied Biosystems Inc. - Forster Ciry CA

International Classification:

C08K 5/1545
C09K 11/00

US Classification:

25230135

Abstract:

Fluorescent polymeric materials are disclosed comprising a polymer and one or more lipid soluble rhodamine dyes. The materials are especially useful in the preparation of multicolored microparticles, especially multicolored polystyrene microparticle, for use in the multiplexed analysis of a plurality of analytes in a single sample. When excited by a light source, the materials give off a unique emission based on the nature, concentration and ratio of the dyes therein. Methods of preparing and using said materials are also disclosed.