Rupert P Amann

US Patent:

6399363, Jun 4, 2002

Filed:

Aug 4, 2000

Appl. No.:

09/635036

Inventors:

Roy H. Hammerstedt - Boalsburg PA
Rupert P. Amann - Fort Collins CO

Assignee:

Biopore, Inc. - State College PA

International Classification:

C12M 136

US Classification:

4352865, 4352971, 4352982, 2103216, 21032165, 21032178, 21032187

Abstract:

An apparatus in which cryoprotectant in cryopreserved samples is removed with the use of turbulence and convective-dispersion around the cells within the primary cryopreservation container. Convective-dispersion is applied via the combined action of gravity and pulsatile transmembrane flow of buffer through the pores of containers fabricated from appropriate membrane material. By using mechanical forces to aid the process of cryoprotectant removal, the benefits of slow cryoprotectant removal are retained while the actual removal time is markedly decreased.

US Patent:

60652942, May 23, 2000

Filed:

Aug 19, 1998

Appl. No.:

9/136552

Inventors:

Roy H. Hammerstedt - Boalsburg PA
Wesley Glebe - State College PA
Rupert P. Amann - Fort Collins CO

Assignee:

BioPore, Inc. - State College PA

International Classification:

F25B 2102

US Classification:

62 33

Abstract:

A long rectangular slotted-sheath construct generally referred to as a "cassette," functions both in the positioning of and the treating of the individual, cell-containing primary units held therein. The invention also embraces a cooling and thawing system within which primary containers of cells, with or without a surrounding cassette, may be cooled, stored and rewarmed.

US Patent:

52618706, Nov 16, 1993

Filed:

Feb 6, 1991

Appl. No.:

7/652295

Inventors:

Roy H. Hammerstedt - Boalsburg PA
Alec D. Keith - Boalsburg PA
Rupert P. Amann - Fort Collins CO

Assignee:

BioPore, Inc. - State College PA

International Classification:

A01N 102

US Classification:

600 35

Abstract:

A noningestible separation barrier having one or more pores or micropores (of one or more diameters) therein, with the pores or micropores being initially plugged with one or more materials selected for its/their solubility and/or integrity characteristics relative to certain environmental conditions. Ordinarily, the pores or micropores of the noningestible separation barrier are initially filled with at least one material having greater erodibility, under a given environmental condition, than the material constituting the separation barrier itself. The combination of the release rate (if any) or other membrane characteristic of the separation barrier, combined with the release rate and/or erosion life of the plugged pores, enables complex separations including variable release of cells, colloids, solutes or solvents over time, such as when the plugged pores remain intact until erosion is triggered by an environmental change such as solvent addition or ionic conditions or pH or thermal change Particular applications include specialized receptacles and protocols for the preservation of rooster sperm and turkey sperm for use in commercial artificial insemination applications.

US Patent:

61240885, Sep 26, 2000

Filed:

Aug 19, 1998

Appl. No.:

9/136556

Inventors:

Rupert P. Amann - Fort Collins CO

Assignee:

BioPore, Inc. - State College PA

International Classification:

A01N 102

US Classification:

435 2

Abstract:

A process and apparatus in which cryoprotectant in cryopreserved samples is removed with the use of turbulence and convective-dispersion around the cells within the primary cryopreservation container. Convective-dispersion is applied via the combined action of gravity and pulsatile transmembrane flow of buffer through the pores of containers fabricated from appropriate membrane material such as that disclosed in U. S. Pat. No. 5,026,342 and U. S. Pat. No. 5,261,870, as incorporated herein by reference above. By using mechanical forces to aid the process of cryoprotectant removal, the benefits of slow cryoprotectant removal are retained while the actual removal time is markedly decreased.

US Patent:

50263420, Jun 25, 1991

Filed:

Mar 14, 1990

Appl. No.:

7/493475

Inventors:

Roy H. Hammerstedt - Boalsburg PA
Alec D. Keith - Boalsburg PA
Rupert P. Amann - Fort Collins CO

Assignee:

Biopore, Inc. - Centre Hall PA

International Classification:

A01N 102
C12N 1104

US Classification:

600 35

Abstract:

A noningestible separation barrier having one or more pores of micropores (of one or more diameters) therein, with the pores or micropores being initially plugged with one or more materials selected for its/their solubility and/or integrity characteristics relative to certain environmental conditions. Ordinarily, the pores or micropores of the noningestible separation barrier are initially filled with at least one material having greater erodibility, under a given environmental condition, than the material constituting the separation barrier itself. The combination of the release rate (if any) or other membrane characteristic of the separation barrier, combined with the release rate and/or erosion life of the plugged pores, enables complex separations including variable release of cells, colloids, solutes or solvents over time, such as when the plugged pores remain intact until erosion is triggered by an environmental change such as solvent addition or ionic conditions or pH or thermal change. Particular applications include specialized receptacles and protocols for the preservation of rooster sperm and turkey sperm for use in commercial artificial insemination applications.