Sally E Webb

US Patent:

20060078960, Apr 13, 2006

Filed:

May 19, 2005

Appl. No.:

11/134850

Inventors:

Christie Hunter - San Mateo CA, US
Sally Webb - Campbell CA, US
Anthony Hunt - Sudbury MA, US
Neil Kitteringham - Merseyside, GB
Stephen Pennington - Co Dublin, IE

International Classification:

C12Q 1/26
G06F 19/00

US Classification:

435025000, 702019000

Abstract:

In various aspects, the present teachings provide systems, methods, assays and kits for the absolute quantitation of protein expression. In various aspects, the present teachings provide methods of determining the concentration of one or more proteins of interest in one or more samples of interest. In various aspects, the present teachings provide methods of determining the absolute concentration of one or more isoforms of a protein using standard samples of signature protein fragments and parent-daughter ion transition monitoring (PDITM). In various embodiments, the absolute concentration of multiple isoforms of a biomolecule in a sample, multiple proteins in a biological process, a combination of multiple samples, or combinations thereof, can be determined in a multiplex fashion using the present teachings. In various aspects, provided are methods of assessing the response of a biological system to a chemical agent.