The present invention provides methods of harvesting rare cells from blood products and/or obtaining products of the rare cells. The method includes contacting a blood product containing rare cells with a porous medium, and selectively retaining rare cells of interest on the porous medium. The porous medium can be contacted with an elution fluid wherein a population of the rare cells is eluted from the porous medium. Rare cells selectively retained on the porous medium can be cultured on the porous medium, and products of the rare cells can be obtained.
Harvey Brandwein - Roslyn Heights NY, US Samuel Coker - Dix Hills NY, US
Assignee:
Pall Corporation - East Hills NY
International Classification:
C12N005/08
US Classification:
435/372000
Abstract:
The present invention provides methods of harvesting rare cells from blood products and/or obtaining products of the rare cells. The method includes contacting a blood product containing rare cells with a porous medium, and selectively retaining rare cells of interest on the porous medium. The porous medium can be contacted with an elution fluid wherein a population of the rare cells is eluted from the porous medium. Rare cells selectively retained on the porous medium can be cultured on the porous medium, and products of the rare cells can be obtained.
Raymond P. Goodrich - Pasadena CA Samuel O.S. Coker - South Pasadena CA Francoise Arnaud - Pasadena CA Roger W. Hackett - Pasadena CA
Assignee:
COBE Laboratories, Inc. - Lakewood CO
International Classification:
A01N 102 C12N 500 G01N 3100
US Classification:
435 2
Abstract:
A process is provided for freezing, including freeze-drying of cells, cell-membranes or cell-like materials using a cryoprotectant medium which stabilizes the cells or membranes for freezing or freeze-drying and allows for freezing or freeze-drying to be performed at -60. degree. C. or higher.