Sarah R Fair

US Patent:

20140178979, Jun 26, 2014

Filed:

Dec 21, 2012

Appl. No.:

13/724316

Inventors:

Joseph HERMAN - West Chester PA, US
Sarah J. FAIR - Manchaug MA, US
Dayana ARGOTI - Charlestown MA, US

International Classification:

G01N 30/02

US Classification:

4352886, 422 70, 422 63

Abstract:

An apparatus comprises: a first chromatographic column fluidically coupled to a source of sample and a source of a first chromatographic mobile phase solvent; a second chromatographic column fluidically coupled to the first column; a source of a second mobile phase solvent fluidically coupled between the first and second columns; and a detector, the first chromatographic column being configurable to receive, in a trapping step, the first solvent and sample and to retain a first portion of a plurality of analytes therein and to pass a second portion of the plurality of analytes therethrough, the second chromatographic column being configurable, in the trapping step, to receive the second portion of the plurality of analytes and the first and second solvents and to retain the second portion of the plurality of analytes therein, the detector being arranged to receive the second and first portions in respective first and second elution steps.