Senthilkumar K Ramaswamy

US Patent:

8137561, Mar 20, 2012

Filed:

Jun 23, 2011

Appl. No.:

13/167297

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

Assignee:

EMD Millipore Corporation - Billerica MA

International Classification:

B01D 15/08

US Classification:

210635, 210656, 2101982, 2105021

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

US Patent:

8177537, May 15, 2012

Filed:

Mar 2, 2010

Appl. No.:

12/660708

Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Nashua NH, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Umana Joaquin - Stoneham MA, US
Neil Soice - Amherst NH, US

Assignee:

EMD Millipore Corporation - Billerica MA

International Classification:

B29B 9/00

US Classification:

425 6, 425 10, 366341

Abstract:

The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads.

US Patent:

8435406, May 7, 2013

Filed:

Feb 1, 2012

Appl. No.:

13/363397

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

Assignee:

EMD Millipore Corporation - Billerica MA

International Classification:

B01D 15/08

US Classification:

2101982, 2105021, 210635, 210656

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities even at high conductivities and pH.

US Patent:

20070069408, Mar 29, 2007

Filed:

Sep 13, 2006

Appl. No.:

11/520775

Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Nashua NH, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin Umana - StoneHame MA, US
Neil Soice - Amherst NH, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01J 13/04
B28B 1/54

US Classification:

264004100, 425005000

Abstract:

The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/ or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads. An apparatus for running the process is also disclosed.

US Patent:

20090050566, Feb 26, 2009

Filed:

Aug 4, 2008

Appl. No.:

12/221496

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

International Classification:

B01D 15/36
B32B 9/00
B05D 3/02

US Classification:

210656, 4283184, 210506, 4273855, 427551

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

US Patent:

20100174051, Jul 8, 2010

Filed:

Mar 2, 2010

Appl. No.:

12/660715

Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Nashua NH, US
Chen Wang - Acton MA, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin A. Umana - Stoneham MA, US
Dennis Aquino - Chelmsford MA, US
Neil Soice - Amherst NH, US
Lyddiatt Andrew - Co Durham, GB

Assignee:

Millipore Corporation - Billerica MA

International Classification:

C07K 1/22

US Classification:

5303881

Abstract:

The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

US Patent:

20100200507, Aug 12, 2010

Filed:

Apr 21, 2010

Appl. No.:

12/764340

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

Assignee:

MILLIPORE CORPORATION - Billerica MA

International Classification:

B01D 15/08

US Classification:

210656

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

US Patent:

20110284446, Nov 24, 2011

Filed:

Jun 23, 2011

Appl. No.:

13/167291

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

Assignee:

MILLIPORE CORPORATIOIN - Billerica MA

International Classification:

B01D 15/36
B01J 41/20
B01D 15/08

US Classification:

2101982, 2105021, 21050027, 21050037

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.