Sergey G Lokhov from 3318 175Th St SE, Bothell, WA 98012, age 70, Phone: (425)8771115

Modified Oligonucleotides For Mismatch Discrimination
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US Patent:

7045610, May 16, 2006
Filed:

Feb 28, 2001
Appl. No.:

09/796988
Inventors:

Robert O. Dempcy - Kirkland WA, US
Alexander A. Gall - Woodland WA, US
Sergey G. Lokhov - Kirkland WA, US
Irina A. Afonina - Mill Creek WA, US
Michael J. Singer - Seattle WA, US
Igor V. Kutyavin - Woodinville WA, US
Nicolaas M. J. Vermeulen - Woodinville WA, US
Assignee:

Epoch Biosciences, Inc. - Bothell WA
International Classification:

C07H 21/00
C07H 21/02
C07H 21/04
C12P 19/34
G01N 31/00
US Classification:

536 231, 536 243, 536 253, 536 266, 435 911, 436 6, 436501
Abstract:

Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.

Real-Time Linear Detection Probes: Sensitive 5′-Minor Groove Binder-Containing Probes For Pcr Analysis
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US Patent:

7205105, Apr 17, 2007
Filed:

Jun 6, 2002
Appl. No.:

10/165410
Inventors:

Irina A. Afonina - Mill Creek WA, US
Yevgeniy S. Belousov - Mill Creek WA, US
Robert O. Dempcy - Kirkland WA, US
Igor V. Kutyavin - Woodinville WA, US
Sergey G. Lokhov - Kirkland WA, US
Eugeny A. Lukhtanov - Bothell WA, US
Assignee:

Epoch Biosciences, Inc. - Bothell WA
International Classification:

C12Q 1/68
C12P 19/34
C07H 21/00
C07H 21/02
C07H 21/04
US Classification:

435 6, 435 911, 435 912, 536 231, 536 266
Abstract:

Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5′ nuclease digestion.

Single Nucleotide Polymorphism Analysis Of Highly Polymorphic Target Sequences
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US Patent:

7348146, Mar 25, 2008
Filed:

Sep 29, 2004
Appl. No.:

10/954955
Inventors:

Yevgeniy Belousov - Mill Creek CA, US
Robert O. Dempcy - Kirkland WA, US
Sergey G. Lokhov - Kirkland WA, US
Alexei Vorobiev - Redmond WA, US
Assignee:

Epoch Biosciences, Inc. - Bothell WA
International Classification:

C12Q 1/68
C12P 19/34
C07H 21/00
C07H 21/02
US Classification:

435 6, 435 911, 536 231, 536 243, 536 2433
Abstract:

Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.

Tleveling Compositions
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US Patent:

7368549, May 6, 2008
Filed:

Apr 19, 2005
Appl. No.:

11/109923
Inventors:

Robert O. Dempcy - Kirkland WA, US
Alexander A Gall - Woodinville WA, US
Sergey G. Lokhov - Kirkland WA, US
Irina A. Afonina - Mill Creek WA, US
Michael J. Singer - Woodinville WA, US
Igor V. Kutyavin - Woodinville WA, US
Nicolaas M. J. Vermeulen - Woodinville WA, US
Assignee:

Epoch Biosciences, Inc. - Bothell WA
International Classification:

C07H 21/00
C07H 21/02
C07H 21/04
C12Q 1/68
US Classification:

536 231, 536 243, 536 2433, 435 6
Abstract:

Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.

Fluorescent Probes Containing 5′-Minor Groove Binder, Fluorophore And Quenching Moieties And Methods Of Use Thereof
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US Patent:

7381818, Jun 3, 2008
Filed:

Oct 27, 2004
Appl. No.:

10/976365
Inventors:

Sergey Lokhov - Kirkland WA, US
Eugene Lukhtanov - Bothell WA, US
Assignee:

Epoch Biosciences, Inc. - Bothell WA
International Classification:

C07H 21/04
C12P 19/34
US Classification:

536 243, 435 912
Abstract:

Minor groove binder-oligonucleotide probes are provided along with methods for their use wherein the probes have an attached fluorophore which, in an unhybridized form exhibits very low background signal.

Real-Time Linear Detection Probes: Sensitive 5'-Minor Groove Binder-Containing Probes For Pcr Analysis
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US Patent:

7485442, Feb 3, 2009
Filed:

Jun 19, 2006
Appl. No.:

11/471292
Inventors:

Irina A. Afonina - Mill Creek WA, US
Yevgeniy S. Belousov - Mill Creek WA, US
Robert O. Dempcy - Kirkland WA, US
Igor V. Kutyavin - Woodinville WA, US
Sergey G. Lokhov - Kirkland WA, US
Eugeny A. Lukhtanov - Bothell WA, US
Assignee:

Epoch Biosciences, Inc. - Bothell WA
International Classification:

C12P 19/34
C12Q 1/68
C07H 21/00
C07H 21/04
US Classification:

435 911, 435 6, 435 912, 536 243, 536 266
Abstract:

Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5′ nuclease digestion.

Systems And Methods For Predicting Oligonucleotide Melting Temperature (Ts)
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US Patent:

7715989, May 11, 2010
Filed:

Jun 18, 2002
Appl. No.:

10/176972
Inventors:

Robert O. Dempcy - Kirkland WA, US
Alexander A. Gall - Woodland WA, US
Sergey G. Lokhov - Kirkland WA, US
Irina A. Afonina - Mill Creek WA, US
Michael J. Singer - Seattle WA, US
Igor V. Kutyavin - Woodinville WA, US
Nicolaas M. J. Vermeulen - Woodinville WA, US
Assignee:

Elitech Holding B.V.
International Classification:

G01N 33/48
US Classification:

702 20
Abstract:

Software systems and methods for predicting the melting temperature (Tm) and other characteristics of oligonucleotides, including modified oligonucleotides. Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.

Single Nucleotide Polymorphism Analysis Of Highly Polymorphic Target Sequences
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US Patent:

7718374, May 18, 2010
Filed:

Jan 28, 2008
Appl. No.:

12/020908
Inventors:

Yevgeniy Belousov - Mill Creek CA, US
Robert O. Dempcy - Kirkland WA, US
Sergey G. Lokhov - Kirkland WA, US
Alexei Vorobiev - Redmond WA, US
Assignee:

Elitech Holding B.V.
International Classification:

C12Q 1/68
C07H 21/00
C12P 19/34
US Classification:

435 6, 435 911, 435 912, 536 231, 536 243, 536 253, 536 266
Abstract:

Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.

Sergey G Lokhov