Seymour P Halbert

US Patent:

44748787, Oct 2, 1984

Filed:

Sep 29, 1975

Appl. No.:

6/617743

Inventors:

Seymour P. Halbert - Miami FL
Milton Anken - N. Miami Beach FL

Assignee:

Cordis Laboratories, Inc. - Miami FL

International Classification:

G01N 3354
G01N 3358

US Classification:

435 7

Abstract:

A sensitive direct immunoassay system is provided for the detection of an antigen associated with hepatitis in body fluids. A single antibody which reacts with a hepatitis antigen or antigens and which is bonded to an insoluble member, is incubated with a test sample. During this first period of incubation a portion of an antigen present in the test sample will combine with the antibody immobilized on the insoluble member. The antibody bonded member, to which antigen is attached, is then washed and incubated with an enzyme tagged antibody reagent. During the second incubation, the tagged antibody reacts with antigen fixed to the antibody member in the first incubation. Thus, an immobilized "sandwich" is formed of an insoluble member- antibody-antigen-enzyme tagged antibody. After the second incubation, the member is washed again to remove unreacted enzyme antibody reagent. The member is then exposed to a substrated which is converted by the enzyme to produce an end product.

US Patent:

46422854, Feb 10, 1987

Filed:

May 2, 1984

Appl. No.:

6/606122

Inventors:

Seymour P. Halbert - Miami FL
Milton Anken - N. Miami Beach FL

Assignee:

Diamedix Corporation - Miami FL

International Classification:

G01N 3353
G01N 33535
G01N 33545
G01N 33576

US Classification:

435 7

Abstract:

A sensitive direct immunoassay system is provided for the detection of an antigen in body fluids. A single antibody which reacts with an antigen or antigens and which is bonded to an insoluble member, is incubated with a test sample. During this first period of incubation a portion of an antigen present in the test sample will combine with the antibody immobilized on the insoluble member. The antibody bonded member, to which antigen is attached, is then washed and incubated with an enzyme tagged antibody reagent. During the second incubation, the tagged antibody reacts with antigen fixed to the antibody member in the first incubation. Thus, an immobilized "sandwich" is formed of an insoluble member- antibody-antigen-enzyme tagged antibody. After the second incubation, the member is washed again to remove unreacted enzyme antibody reagent. The member is then exposed to a substrate which is converted by the enzyme to produce an end product.

US Patent:

41572807, Jun 5, 1979

Filed:

Sep 29, 1975

Appl. No.:

5/617745

Inventors:

Seymour P. Halbert - Miami FL
Milton Anken - North Miami Beach FL

Assignee:

Cordis Corporation - Miami FL

International Classification:

G01N 3114
G01N 3316

US Classification:

195127

Abstract:

A test set useful for detecting the presence of antigens associated with hepatitis comprising an insoluble, polymeric disc-like solid having antibody reactive with antigens associated with hepatitis bonded thereto, an enzyme-hepatitis antibody conjugate reagent, an enzyme substrate solution, control sera negative, weakly positive, and strongly positive for antigens associated with hepatitis, an enzyme substrate buffer, and a horse globulin solution. Preferably, the enzyme of the conjugate is alkaline phosphatase, the enzyme substrate is p-nitrophenylphosphate and the substrate buffer has a pH on the order of 9. 8.

US Patent:

43178103, Mar 2, 1982

Filed:

Mar 2, 1980

Appl. No.:

6/126525

Inventors:

Seymour P. Halbert - Miami FL
Milton Anken - N. Miami Beach FL

Assignee:

Cordis Laboratories, Inc. - Miami FL

International Classification:

G01N 3106
G01N 3348
G01N 3352
G01N 3354

US Classification:

424 12

Abstract:

A disc-shaped insoluble matrix is formed of a polymeric material having a layer of reactive groups which react with proteins binding them covalently and uniformly to its surface. An antibody adapted for use in immunoassay of antigens associated with hepatitis is covalently bonded to the reactive groups. The matrix is provided with irregular, e. g. , waffle-like, surfaces designed to increase the amount of antibody that will be in contact with a test solution such as serum.

US Patent:

46378804, Jan 20, 1987

Filed:

Jun 1, 1984

Appl. No.:

6/616789

Inventors:

Seymour P. Halbert - Miami FL

Assignee:

Cordis Laboratories, Inc. - Miami FL

International Classification:

B01D 1300

US Classification:

210638

Abstract:

A therapeutic apparatus for the removal of a harmful agent from blood is provided in the form of an array of hollow fiber bundles connected in parallel, wherein the interior surfaces of the individual hollow fibers are coated with a covalently bound proteinaceous or other immunoadsorbent selected for specific removal of the harmful agent. In the disclosed method the apparatus is optionally connected as an extracorporeal shunt between the artery and vein of a patient to be treated and the blood is alternately routed through one fiber bundle while the remaining fiber bundles undergo regeneration. Specific embodiments include the removal of rheumatoid factor by covalently bound immunoglobulin G and the removal of digoxin by covalently bound anti-digoxin.

US Patent:

62651765, Jul 24, 2001

Filed:

Feb 6, 1995

Appl. No.:

8/384681

Inventors:

Seymour P. Halbert - Miami FL

Assignee:

Cordis Corporation - Miami FL

International Classification:

G01N 33543

US Classification:

435 792

Abstract:

Analytical test kits and assay procedure employs test cards or strips of an inert plastic carrier having a flat, non-absorbent surface, containing thereon spots of insolubilized reagent for the determination of an analyte, such as an antigen or an antibody, in a liquid medium such as serum or other body fluids in a solid phase enzyme immunoassay of the sandwich type. Different binding partners can be placed as separate spots on the same card for the assay of a group of related or unrelated analytes in the same test sample. An insoluble colored product on a spot, qualitatively and quantitatively, is indicative of a positive result.