Shannon L Mitchell

US Patent:

20020115208, Aug 22, 2002

Filed:

Aug 16, 2001

Appl. No.:

09/931506

Inventors:

Shannon Mitchell - Durham NC, US
Jennifer Koh - Irvine CA, US
Vikas Prabhakar - Boston MA, US
Laura Niklason - Hillsborough NC, US

International Classification:

C12N005/06
C12N005/00

US Classification:

435/325000, 435/402000

Abstract:

New methods for producing tissue engineered constructs and engineered native tissues are disclosed. The methods include producing a tissue engineered construct by growing cells in vitro on a substrate and then decellularizing the construct to produce a decellularized construct consisting largely of extracellular matrix components. The construct can be used immediately or stored until needed. The decellularized construct can be used for further tissue engineering, which may include seeding the construct with cells obtained from the intended recipient of the construct. During any of the growth phases required for production of the construct, the developing construct may be subjected to various tissue engineering steps such as application of mechanical stimuli including pulsatile forces. The methods also include producing an engineered native tissue by harvesting tissue from an animal or human, performing one or more tissue engineering steps on the tissue, and subjecting the tissue to decellularization. The decellularized, engineered native tissue may then be subjected to further tissue engineering steps.

US Patent:

20060073590, Apr 6, 2006

Filed:

Oct 19, 2005

Appl. No.:

11/252993

Inventors:

Shannon Mitchell - Durham NC, US
Jennifer Koh - Irvine CA, US
Vikas Prabhakar - Cambridge MA, US
Laura Niklason - Hillsborough NC, US

Assignee:

Duke University - Durham NC

International Classification:

C12N 5/00

US Classification:

435402000

Abstract:

New methods for producing tissue engineered constructs and engineered native tissues are disclosed. The methods include producing a tissue engineered construct by growing cells in vitro on a substrate and then decellularizing the construct to produce a decellularized construct consisting largely of extracellular matrix components. The construct can be used immediately or stored until needed. The decellularized construct can be used for further tissue engineering, which may include seeding the construct with cells obtained from the intended recipient of the construct. During any of the growth phases required for production of the construct, the developing construct may be subjected to various tissue engineering steps such as application of mechanical stimuli including pulsatile forces. The methods also include producing an engineered native tissue by harvesting tissue from an animal or human, performing one or more tissue engineering steps on the tissue, and subjecting the tissue to decellularization. The decellularized, engineered native tissue may then be subjected to further tissue engineering steps.

US Patent:

20080248080, Oct 9, 2008

Filed:

May 21, 2008

Appl. No.:

12/124521

Inventors:

Shannon Mitchell - Durham NC, US
Jennifer Koh - Irvine CA, US
Vikas Prabhakar - Cambridge MA, US
Laura Niklason - Hillsborough NC, US

Assignee:

Duke University - Durham NC

International Classification:

A61F 2/00
C12N 5/06
A61K 35/34
G01N 33/53
C07K 1/00
A61P 19/00

US Classification:

424423, 435395, 424548, 435 721, 435272

Abstract:

New methods for producing tissue engineered constructs and engineered native tissues are disclosed. The methods include producing a tissue engineered construct by growing cells in vitro on a substrate and then decellularizing the construct to produce a decellularized construct consisting largely of extracellular matrix components. The construct can be used immediately or stored until needed. The decellularized construct can be used for further tissue engineering, which may include seeding the construct with cells obtained from the intended recipient of the construct. During any of the growth phases required for production of the construct, the developing construct may be subjected to various tissue engineering steps such as application of mechanical stimuli including pulsatile forces. The methods also include producing an engineered native tissue by harvesting tissue from an animal or human, performing one or more tissue engineering steps on the tissue, and subjecting the tissue to decellularization. The decellularized, engineered native tissue may then be subjected to further tissue engineering steps.

US Patent:

20120135049, May 31, 2012

Filed:

Dec 28, 2011

Appl. No.:

13/338847

Inventors:

Shannon MITCHELL - Morrisville NC, US
Jennifer KOH - Irvine CA, US
Vikas PRABHAKAR - Cambridge MA, US
Laura NIKLASON - Greenwich CT, US

Assignee:

DUKE UNIVERSITY - Durham NC

International Classification:

A61K 9/00
A61K 35/12

US Classification:

424400, 424 937

Abstract:

Methods for producing tissue engineered constructs and engineered native tissues include producing a tissue engineered construct by growing cells in vitro on a substrate and then decellularizing the construct to produce a decellularized construct consisting largely of extracellular matrix components. The construct can be used immediately or stored until needed. The decellularized construct can be used for further tissue engineering, which may include seeding the construct with cells obtained from the intended recipient of the construct. During any of the growth phases required for production of the construct, the developing construct may be subjected to various tissue engineering steps such as application of mechanical stimuli including pulsatile forces.