Shaojing Chang

US Patent:

20100183628, Jul 22, 2010

Filed:

Jan 6, 2010

Appl. No.:

12/652877

Inventors:

Michael S. Kinch - Laytonsville MD, US
Michael Goldblatt - McLean VA, US
Douty Bamba - Randallstown MD, US
Shaojing Chang - Rockville MD, US
Huosheng Chen - Germantown MD, US
Zenbework Fesseha - Columbia MD, US
Manu Kohli - Dunn Loring VA, US
Hanwen Mao - Annandale VA, US
Heather Thi Thu Ung-Medoff - Gaithersburg MD, US
Ke Weng - Gaithersburg MD, US

Assignee:

FUNCTIONAL GENETICS, INC. - GAITHERSBURG MD

International Classification:

A61K 39/395
C12Q 1/70
A61P 31/16
A61P 31/18

US Classification:

4241721, 435 5

Abstract:

A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of the genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is rapidly verifiable and is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL)-PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.

US Patent:

20140271671, Sep 18, 2014

Filed:

Mar 14, 2013

Appl. No.:

13/803877

Inventors:

- Indianapolis IN, US
Michael Goldblatt - McLean VA, US
Wu-Bo Li - North Potomac MD, US
Douty Bamba - Randallstown MD, US
Shaojing Chang - Rockville MD, US
Huosheng Chen - Germantown MD, US
Zenbework Fesseha - Columbia MA, US
Manu Kohli - Dunn Loring VA, US
Hanwen Mao - Annandale VA, US
Heather Thi Thu Ung-Medoff - Gaithersburg MD, US
Ke Weng - Gaithersburg MD, US

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12Q 1/68

US Classification:

4241721, 435 5, 435375

Abstract:

A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of the genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is rapidly verifiable and is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL)-PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.