Stephanie M Yeung

US Patent:

20120142010, Jun 7, 2012

Filed:

Feb 13, 2012

Appl. No.:

13/372376

Inventors:

Richard A. MATHIES - San Francisco CA, US
Robert BLAZEJ - San Francisco CA, US
Chung LIU - Albany CA, US
Palani KUMARESAN - Los Angeles CA, US
Stephanie H.I. YEUNG - Berkeley CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12Q 1/68

US Classification:

435 612

Abstract:

Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments. The microfabricated structure may be used in a process for performing sequencing and other genetic analysis of DNA or RNA.

US Patent:

20050287572, Dec 29, 2005

Filed:

May 25, 2005

Appl. No.:

11/139018

Inventors:

Richard Mathies - Moraga CA, US
Robert Blazej - Berkeley CA, US
Chung Liu - Albany CA, US
Palani Kumaresan - Berkeley CA, US
Stephanie Yeung - Berkeley CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q001/68
C12M001/34

US Classification:

435006000, 435287200

Abstract:

Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments. The microfabricated structure may be used in a process for performing sequencing and other genetic analysis of DNA or RNA.