Stephen W Adams

US Patent:

61804118, Jan 30, 2001

Filed:

Jul 30, 1998

Appl. No.:

9/134668

Inventors:

Roger Y. Tsien - La Jolla CA
Stephen R. Adams - Poway CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 33532
G01N 33554
C07D30782
C07D491048

US Classification:

436 79

Abstract:

A new class of optical indicators which are capable of memorizing and preserving the spatial localization of intracellular analytes in a time resolved manner is described. The compounds comprise a chromophore carrying a photolabile group capable of undergoing an irreversible and detectable chemical transformation upon irradiation by light. The chromophore is linked to a binding site capable of binding an analyte, wherein binding of the analyte to the binding site alters an optical property of the chromophore, thus altering the ability of the photolabile group to undergo the chemical transformation. Methods and kits for memorizing the spatial localization of the analytes are also described.

US Patent:

54397979, Aug 8, 1995

Filed:

Aug 30, 1993

Appl. No.:

8/114103

Inventors:

Roger Y. Tsien - La Jolla CA
Susan S. Taylor - Del Mar CA
Stephen R. Adams - Poway CA
Ying Ji - San Diego CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 33533

US Classification:

435 721

Abstract:

The invention provides labeled proteins suitable for determining the presence of cAMP, other second messengers and organic molecules. The proteins are separately labeled with fluorochromes which, when in close spatial proximity, preferably, less than about 6 nm, interact through the transfer of energy from one fluorochrome to the other. A composition of matter, (S. sub. 1. A). sub. n1 (S. sub. 2. D). sub. n2 is provided wherein S. sub. 1 and S. sub. 2 are two proteins which are associated in one state and substantially disassociated in another, the equilibrium between which is controlled by the free concentration of an analyte, and A and D are fluorochromes, the emission wavelength of fluorochrome D overlapping the excitation wavelength of fluorochrome A and the distance between A and D being in sufficiently close proximity to allow the radiationless transfer of energy between the fluorochromes. A and D can be selected from a variety of acceptor, donor pairs such as fluorescein and tetramethylrhodamine and derivatives thereof. The concentration of analytes such as cAMP in a sample can be determined by contacting the sample with (S. sub. 1. A). sub. n1 (S. sub. 2. D). sub.

US Patent:

56022581, Feb 11, 1997

Filed:

Oct 23, 1992

Appl. No.:

7/968002

Inventors:

Roger Y. Tsien - La Jolla CA
Stephen R. Adams - Poway CA

Assignee:

The Regents of University of California - Oakland CA

International Classification:

C07D26330

US Classification:

548235

Abstract:

The present invention relates to a group of organic chelators whose affinity for calcium ion in solution is decreased by electromagnetic radiation. Specifically, the chelators are related to fura-2 and utilize the addition of an azide group to the 3-position of the benzofuron ring of a fura-2 type structure. Photolysis of the azide group causes the calcium ion affinity to decrease 100 to 1000 fold. These chelators when incorporated into rat fibroblasts either by microinjection or by incubation as the membrane-permeable, enzymatically-labile esters and flash-photolyzed cause large increases in intracellular free calcium ion. These chelators are used to generate controlled fast elevation of intracellular free calcium ion concentration to mimic or modulate a number of important cellular responses, especially in nerve or muscle.

US Patent:

55525555, Sep 3, 1996

Filed:

Jun 6, 1995

Appl. No.:

8/475047

Inventors:

Roger Y. Tsien - La Jolla CA
Stephen R. Adams - Poway CA

Assignee:

The Regents of University of California - Oakland CA

International Classification:

C07D41314

US Classification:

548236

Abstract:

The present invention relates to a group of organic chelators whose affinity for calcium ion in solution is decreased by electromagnetic radiation. Specifically, the chelators are related to fura-2 and utilize the addition of an azide group to the 3-position of the benzofuran ring of a fura-2 type structure. Photolysis of the azide group causes the calcium ion affinity to decrease 100 to 1000 fold. These chelators when incorporated into rat fibroblasts either by microinjection or by incubation as the membrane-permeable, enzymatically-labile esters and flash-photolyzed cause large increases in intracellular free calcium ion. These chelators are used to generate controlled fast elevation of intracellular free calcium ion concentration to mimic or modulate a number of important cellular responses, especially in nerve or muscle.

US Patent:

51416271, Aug 25, 1992

Filed:

Sep 25, 1989

Appl. No.:

7/412852

Inventors:

Roger Y. Tsien - La Jolla CA
Stephen R. Adams - San Diego CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07C24508
C07C 4500

US Classification:

20415788

Abstract:

The present invention relates to a group of organic chelators whose affinity for calcium ion in solution is increased by electromagnetic radiation. Specifically, the chelators are related to BAPTA and utilize the addition of an electron-withdrawing group (e. g. , diazocarbonyl) to a ring of BAPTA, para to the amino group. Photochemical rearrangement of the diazoacetyl group converts the group to the electron-donating carboxymethyl group, causing the calcium ion efficiency to increase 25 to 50 fold. These chelators when incorporated into rat fibroblasts either by microinjection or by incubation as the membrane-permeable, enzymatically-labile tetraacetoxymethyl ester and flash-photolyzed cause a drop in intracellular free calcine ion to or below resting valves of about 10. sup. -7 M. These chelators are used to generate controlled fast removal of intracellular free calcium ion to mimic or modulate a number of important cellular responses, especially in nerve or muscle.

US Patent:

20220288223, Sep 15, 2022

Filed:

Mar 12, 2020

Appl. No.:

17/438364

Inventors:

- Oakland CA, US
Stephen Adams - Poway CA, US
Qing Xiong - San Diego CA, US

International Classification:

A61K 47/68
A61K 47/60
C07K 16/28

Abstract:

Disclosed herein, the invention pertains to methods and compositions involving activatable specific binding member complex(es).

US Patent:

20220273824, Sep 1, 2022

Filed:

Aug 6, 2020

Appl. No.:

17/630286

Inventors:

- Oakland CA, US
Dina Hingorani - San Diego CA, US
Stephen Adams - Poway CA, US

International Classification:

A61K 49/00
A61K 49/18
A61B 6/03
A61B 6/00
C07K 14/16

Abstract:

This disclosure provides compositions of fluorinated nanoemulsions and associated methods for producing cellular labels for tracking cells by magnetic resonance imaging (MRI), computed tomography (CT), positron emission tomography (PET), and related methods.

US Patent:

20210363186, Nov 25, 2021

Filed:

May 21, 2021

Appl. No.:

17/326339

Inventors:

- Oakland CA, US
Mike Whitney - San Diego CA, US
Dina Hingorani - San Diego CA, US
Roger Y. Tsien - Eugene OR, US
Stephen Adams - Poway CA, US

International Classification:

C07K 7/08
A61K 47/64
A61K 9/00
A61K 41/00
A61K 49/00
C07K 7/00
G01N 33/50

Abstract:

The present invention provides methods for guiding preservation of human neurons or human nerves during surgery by administering a fluorescently-labeled peptide that specifically binds to the human neurons or human nerves. The invention further provides human neuron or nerve targeting molecules comprising fluorescently-labeled peptides that specifically bind to human neurons or human nerves and compositions thereof.