Stephen W Hendricks

US Patent:

20110159551, Jun 30, 2011

Filed:

Oct 8, 2010

Appl. No.:

12/900794

Inventors:

Roland Nagel - Santa Cruz CA, US
Stephen Hendricks - Los Gatos CA, US
Jennifer Berkman - San Francisco CA, US
Marian Peris - Belmont CA, US
Yulei Wang - Foster City CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 19/34
C12N 9/10

US Classification:

435 915, 435193

Abstract:

The present invention provides compositions and methods for a reverse transcription reaction using a reversibly inactivated reverse transcriptase enzyme. The reversibly inactivated reverse transcriptase enzyme results from a chemical modification which inactivates the reverse transcriptase enzyme. The activity of the reverse transcriptase enzyme is recovered by an incubation of the reaction mixture at elevated temperature prior to, or as part of the reverse transcription reaction. The reverse transcriptase enzyme of the present invention provides for a signficant reduction in non-specific reverse transcription from template nucleic acid molecules because the formulation of the reaction mixture does not support the formation of reverse transcription products prior to activation of the reverse transcriptase.

US Patent:

20170044506, Feb 16, 2017

Filed:

Aug 22, 2016

Appl. No.:

15/243808

Inventors:

- Carlsbad CA, US
Roland Nagel - Santa Cruz CA, US
Stephen Hendricks - Los Gatos CA, US
Jennifer Berkman - San Francisco CA, US
Marian Peris - San Mateo CA, US
Yulei Wang - Foster City CA, US

International Classification:

C12N 9/12
C12N 15/10

Abstract:

The present invention provides compositions and methods for a reverse transcription reaction using a reversibly inactivated reverse transcriptase enzyme. The reversibly inactivated reverse transcriptase enzyme results from a chemical modification which inactivates the reverse transcriptase enzyme. The activity of the reverse transcriptase enzyme is recovered by an incubation of the reaction mixture at elevated temperature prior to, or as part of the reverse transcription reaction. The reverse transcriptase enzyme of the present invention provides for a significant reduction in non-specific reverse transcription from template nucleic acid molecules because the formulation of the reaction mixture does not support the formation of reverse transcription products prior to activation of the reverse transcriptase.