Stephen John Kent

US Patent:

20060121535, Jun 8, 2006

Filed:

Dec 16, 2005

Appl. No.:

11/305671

Inventors:

Shawn Brueggemeier - East Windsor NJ, US
Stephen Kron - Oak Park IL, US
Sean Palecek - Madison WI, US
Laurie Parker - Chicago IL, US
Stephen Kent - San Francisco CA, US

International Classification:

G01N 33/53
C12Q 1/37
G01N 33/00

US Classification:

435007100, 435023000, 436086000

Abstract:

Disclosed are a polyacrylamide-based method of fabricating surface-bound peptide and protein arrays, the arrays themselves, and a method of using the arrays to detect biomolecules and to measure their concentration, binding affinity, and kinetics. Peptides, proteins, fusion proteins, protein complexes, nucleic acids, and the like, are labeled with an acrylic moiety and attached to acrylic-functionalized glass surfaces through a copolymerization with acrylic monomer. The specific attachment of GST-green fluorescent protein (GFP) fusion protein was more than 7-fold greater than the nonspecific attachment of non-acrylic labeled GST-GFP. Surface-attached GST-GFP (0.32 ng/mm) was detectable by direct measurement of GFP fluorescence and this lower detection limit was reduced to 0.080 ng/mmusing indirect antibody-based detection. The polyacrylamide-based surface attachment strategy was also used to measure the kinetics of substrate phosphorylation by the kinase c-Src. The surface attachment strategy is applicable to the proteomics field and addresses denaturation and dehydration problems associated with protein microarray development.