Stephen D Kent

US Patent:

7674881, Mar 9, 2010

Filed:

Oct 10, 2006

Appl. No.:

11/545923

Inventors:

Stephen Kent - Chicago IL, US
Brad Pentelute - Chicago IL, US
Duhee Bang - Boston MA, US
Erik Johnson - Chicago IL, US
Thomas Durek - Chicago IL, US

Assignee:

The University of Chicago - Chicago IL

International Classification:

C07K 1/06
C07K 1/107

US Classification:

530339, 530345

Abstract:

The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal with a second peptide having a cysteine residue at its N-termini and a thioester at its C-termini to form a ligation product. Subsequent reactions may involve deprotecting the cysteyl group of the resulting ligation product and/or converting the thioester into a thiophenylester.