Stephen L Kent

US Patent:

20020051996, May 2, 2002

Filed:

Aug 31, 1998

Appl. No.:

09/144838

Inventors:

MICHAEL A. SIANI - SAN FRANCISCO CA, US
JILL WILKEN - SAN FRANCISCO CA, US
REYNA SIMON - LOS GATOS CA, US
STEPHEN B.H. KENT - SAN FRANCISCO CA, US

International Classification:

C12Q001/00
G01N033/53
C07K014/00

US Classification:

435/007100, 435/004000, 530/350000, 530/324000

Abstract:

Novel proteins and libraries comprising them are disclosed. The proteins comprise one or more functional protein modules from different parent protein molecules. The proteins and libraries are exemplified by the preparation of cross-over chemokines comprising various combinations of peptide segments derived from RANTES, SDF-1 and vMIP-I and vMIP-II. The proteins and libraries are extremely pure and can be provided in non-limiting high yields suitable for diagnostic and high-throughput screening assays.

US Patent:

20020115612, Aug 22, 2002

Filed:

Feb 8, 2002

Appl. No.:

10/071577

Inventors:

Ronald Zuckermann - El Cerrito CA, US
Janice Kerr - Oakland CA, US
Stephen Kent - La Jolla CA, US

Assignee:

Chiron Corporation

International Classification:

A61K038/16
C07K014/00

US Classification:

514/012000, 530/350000

Abstract:

An automated solid-phase method for the synthesis of poly (N-substituted glycines) (referred to herein as poly NSGs) taught here can be used to obtain poly NSGs of potential therapeutic interest which poly NSGs can have a wide variety of side chain substituents. Each N-substituted glycine monomer is assembled from two “sub-monomers” directly on the solid support. Each cycle of monomer addition consists of two steps: (1) acylation of a resin-bound secondary amine with an agent such as a haloacetic acid, and (2) introduction of the side-chain by nucleophilic displacement of the halogen (as a resin-bound -haloacetamide) with an excess of primary amine. The efficient synthesis of a wide variety of oligomeric NSGs using automated synthesis technology, as presented here, makes these polymers attractive candidates for the generation and rapid screening of diverse peptidomimetic libraries. The oligomers of N-substituted glycines (i.e. poly NSGs) disclosed here provide a new class of polymers not found in nature, but which are synthetically accessible and have been shown to possess significant biological activity and proteolytic stability.

US Patent:

20020132975, Sep 19, 2002

Filed:

Nov 15, 2001

Appl. No.:

09/987655

Inventors:

Lynne Canne - Pacifica CA, US
Stephen Kent - San Francisco CA, US
Reyna Simon - Los Gatos CA, US

Assignee:

Gryphon Sciences

International Classification:

C07K016/00

US Classification:

530/324000

Abstract:

The present invention provides methods, apparatus and kits for synthesizing assembled peptides and proteins on a solid phase with sequential ligation of three or more unprotected peptide segments using chemoselective and mild ligation chemistries in aqueous solution. Also provided are methods of monitoring solid phase sequential ligation reactions using MALDI or electrospray ionization mass spectrometry of reaction products.

US Patent:

20020169282, Nov 14, 2002

Filed:

Nov 15, 2001

Appl. No.:

09/987675

Inventors:

Lynne Canne - Pacifica CA, US
Stephen Kent - San Francisco CA, US
Reyna Simon - Los Gatos CA, US

Assignee:

Gryphon Sciences

International Classification:

C07K001/04

US Classification:

530/334000

Abstract:

The present invention provides methods, apparatus and kits for synthesizing assembled peptides and proteins on a solid phase with sequential ligation of three or more unprotected peptide segments using chemoselective and mild ligation chemistries in aqueous solution. Also provided are methods of monitoring solid phase sequential ligation reactions using MALDI or electrospray ionization mass spectrometry of reaction products.

US Patent:

20030018169, Jan 23, 2003

Filed:

Jul 30, 2002

Appl. No.:

10/207330

Inventors:

Gerd Kochendoerfer - Oakland CA, US
Christie Hunter - San Francisco CA, US
Stephen Kent - San Francisco CA, US
Paolo Botti - San Francisco CA, US

International Classification:

C07K014/705

US Classification:

530/350000, 530/408000, 530/409000

Abstract:

The present invention relates to methods and compositions for lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides that are incorporated in a lipid matrix. The invention is exemplified in production of a prefolded membrane polypeptide embedded within a lipid matrix via stepwise chemoselective chemical ligation of unprotected peptide segments, where at least one peptide segment is embedded in a lipid matrix. Any chemoselective reaction chemistry amenable for ligation of unprotected peptide segments can be employed. Suitable lipid matrices include liposomes, micelles, cell membrane patches and optically isotropic cubic lipidic phase matrices. Prefolded synthetic and semi-synthetic membrane polypeptides synthesized according to the methods and compositions of the invention also permit site-specific incorporation of one or more detectable moieties, such as a chromophore, which can be conveniently introduced during synthesis. The methods and compositions of the invention have multiple uses. For example, they can be used to assay ligand binding to membrane polypeptides and domains comprising a receptor, and thus are extremely useful for structure/function studies, drug screening/selection/design, and diagnostics and the like, including high-throughput applications. The methods and compositions of the invention are particularly suited for FRET analyses of previously inaccessible membrane polypeptides.

US Patent:

20030059952, Mar 27, 2003

Filed:

Apr 5, 2001

Appl. No.:

09/828326

Inventors:

Brian Chait - New York NY, US
Ronald Beavis - St. John's, CA
Rong Wang - New York NY, US
Stephen Kent - La Jolla CA, US

International Classification:

G01N033/543
G01N033/00

US Classification:

436/518000, 436/086000

Abstract:

Method is described for sequencing polypeptides by forming peptide ladders comprising a series of polypeptides in which adjacent members of the series vary by one amino acid residue and determining the identity and position of each amino acid in the polypeptide by mass spectroscopy.

US Patent:

20030149234, Aug 7, 2003

Filed:

Jan 9, 2003

Appl. No.:

10/332454

Inventors:

Paolo Botti - Piacenza, IT
James Bradburne - Redwood City CA, US
Stephen Kent - San Francisco CA, US

International Classification:

C07K007/00
C07C327/02

US Classification:

530/300000, 558/250000

Abstract:

The invention is directed to nucleophile-stable thioester generating compounds comprising an orthothioloester or a carboxyester thiol, methods of production and use. The compounds and methods have wide applicability in organic synthesis, including the generation of peptide-, polypeptide- and other polymer-thioesters. The invention is particularly useful for generating activated-thioesters from precursors that are made under conditions in which strong nucleophiles are employed, such as peptides or polypeptides made using Fmoc SPPS, as well as multi-step ligation or conjugation schemes that require (or benefit from the use of) compatible selective approaches for directing a specific ligation or conjugation reaction of interest.

US Patent:

20030191291, Oct 9, 2003

Filed:

Jan 13, 2003

Appl. No.:

10/332696

Inventors:

Gerd Kochendoerfer - Oakland CA, US
Paolo Botti - Piacenza, IT
James Bradburne - Redwood City CA, US
Sonya Cressman - Ladysmith, CA
Christine Hunter - San Mateo CA, US
Stephen Kent - San Francisco CA, US
Donald Low - Burlingame CA, US

International Classification:

C07K014/575

US Classification:

530/397000

Abstract:

Synthetic erythropoiesis stimulating proteins are provided. Also provided are methods for synthesizing the proteins. The invention further relates to derivatives of such synthetic erythropoiesis stimulating proteins that are polymer-modified in a defined manner. Methods and uses for such proteins and derivatized proteins are also provided.