Stephen M Roth

US Patent:

6379933, Apr 30, 2002

Filed:

Jun 24, 1999

Appl. No.:

09/338943

Inventors:

Karl F. Johnson - Willow Grove PA
Stephen Roth - Gladwyne PA
Stephanie L. Buczala - Jenkintown PA

Assignee:

Neose Technologies, Inc. - Horsham PA

International Classification:

C12P 1918

US Classification:

435 97, 435 72, 435 84, 435100, 435101, 435170, 435871

Abstract:

The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.

US Patent:

6518051, Feb 11, 2003

Filed:

May 12, 2000

Appl. No.:

09/570236

Inventors:

Stephen Roth - Gladwyne PA

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C12P 702

US Classification:

435155, 475131, 475 41

Abstract:

A method for preparing saccharide compositions is disclosed. The method is reiterative and includes the following three steps. (i) A glycosyltransferase capable of transferring a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate. (ii) The isolated glycosyltransferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit. (iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.

US Patent:

6544778, Apr 8, 2003

Filed:

Dec 3, 2001

Appl. No.:

10/007286

Inventors:

Stephen Roth - Gladwyne PA

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C12M 134

US Classification:

435288, 435 97, 435819

Abstract:

A method for preparing saccharide compositions is disclosed. The method is reiterative and comprises the following three steps. (i) A glycosyltransferase capable of transferring a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate. (ii) The isolated glycosyltransferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit. (iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.

US Patent:

6569649, May 27, 2003

Filed:

Jun 7, 1995

Appl. No.:

08/484885

Inventors:

Stephen Roth - Gladwyne PA

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C12P 1918

US Classification:

435 97, 435 84, 435 681, 435193, 435814, 435815

Abstract:

Processes for preparing oligosaccharides, polysaccharides, glycolipids, glycoproteins, and other saccharide compositions are provided which involve the enzyme facilitated transfer of a preselected saccharide unit from a donor moiety to an acceptor moiety. In accordance with a preferred embodiment, saccharide compositions having a plurality of saccharide units are prepared by appending the saccharide units in iterative fashion to acceptor moieties which are themselves saccharide compositions prepared in accordance with this invention.

US Patent:

7923445, Apr 12, 2011

Filed:

Aug 31, 2007

Appl. No.:

11/897669

Inventors:

Stephen Roth - Gladwyne PA, US
Clayton Buck - Berwyn PA, US
Christopher Self - West Caldwell NJ, US
Gary Olson - Mountainside NJ, US

Assignee:

Immune Control, Inc. - West Conshohocken PA

International Classification:

A01K 31/54
A01K 31/497

US Classification:

5142222, 5142245, 51425408, 51425301

Abstract:

The present invention relates to inhibiting proliferation and inducing apoptosis in activated lymphocytes, including T cells and B cells. The invention also provides compositions and methods for inhibiting proliferation and inducing apoptosis in activated lymphocytes, as well methods for treating diseases associated with activated lymphocytes by administering 5-HT receptor antagonists.

US Patent:

7981885, Jul 19, 2011

Filed:

Aug 31, 2007

Appl. No.:

11/897598

Inventors:

Stephen Roth - Gladwyne PA, US
Clayton Buck - Berwyn PA, US
Christopher Self - West Caldwell NJ, US
Gary Olson - Mountainside NJ, US

Assignee:

Immune Control Inc. - West Conshohocken PA

International Classification:

C07D 419/06
A61K 31/5415

US Classification:

5142258, 544 42, 544 44

Abstract:

The present invention relates to inhibiting proliferation and inducing apoptosis in activated lymphocytes, including T cells and B cells. The invention also provides compositions and methods for inhibiting proliferation and inducing apoptosis in activated lymphocytes, as well methods for treating diseases associated with activated lymphocytes by administering 5-HT receptor antagonists.

US Patent:

20030130175, Jul 10, 2003

Filed:

Dec 23, 2002

Appl. No.:

10/328625

Inventors:

Stephen Roth - Gladwyne PA, US

Assignee:

The Trustees of the University of Pennsylvania

International Classification:

A61K038/14
A61K038/16
A61K031/715
C12P021/06
C12P019/30
C12P019/04

US Classification:

514/008000, 514/054000, 435/068100, 435/089000, 435/101000

Abstract:

A method for preparing saccharide compositions is disclosed. The method is reiterative and comprises the following three steps. (i) A glycosyltransferase capable of transferring a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate. (ii) The isolated glycosyltransferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit. (iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.

US Patent:

20030207406, Nov 6, 2003

Filed:

Mar 7, 2002

Appl. No.:

10/096129

Inventors:

Karl Johnson - Willow Grove PA, US
Stephen Roth - Gladwyne PA, US
Stephanie Buczala - Jenkintown PA, US

Assignee:

Neose Technologies, Inc.

International Classification:

C12P019/18
C12N009/10

US Classification:

435/097000, 435/193000

Abstract:

The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.