Steven J Ekenberg

US Patent:

20060105349, May 18, 2006

Filed:

Nov 12, 2004

Appl. No.:

10/987514

Inventors:

Steven Ekenberg - Mount Horeb WI, US
Keith Wood - Mount Horeb WI, US
Laurie Engel - DeForest WI, US

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 1/68
G01N 33/53
C12M 1/34

US Classification:

435006000, 435007100, 435287200

Abstract:

An apparatus, method and kit for isolating a biomolecule from a sample. The sample comprises a complex biological material, which includes insoluble matter. Some embodiments of the apparatus and kit include a reservoir and means for capturing the biomolecule either contained within or coupled to the reservoir. The reservoir can have an inner surface, and can be adapted to contain the sample. The apparatus can further include least one of a filter positioned between the means for capturing the biomolecule and at least a portion of the inner surface of the reservoir, and an aperture defined in the inner surface of the reservoir. Some embodiments of the method include combining the sample with a solid phase that is adapted to capture the biomolecule, removing the insoluble matter from the sample, and removing the biomolecule from the solid phase.

US Patent:

20060281124, Dec 14, 2006

Filed:

Aug 24, 2006

Appl. No.:

11/466945

Inventors:

Steven Ekenberg - Mount Horeb WI, US
Keith Wood - Mount Horeb WI, US
Laurie Engel - DeForest WI, US

Assignee:

Promega Corporation - Madison WI

International Classification:

C12Q 1/68
C12M 1/34
G01N 33/558

US Classification:

435006000, 435287200, 436514000

Abstract:

An apparatus, method and kit for isolating a biomolecule from a sample. The sample comprises a complex biological material, which includes insoluble matter. Some embodiments of the apparatus and kit include a reservoir and means for capturing the biomolecule either contained within or coupled to the reservoir. The reservoir can have an inner surface, and can be adapted to contain the sample. The apparatus can further include least one of a filter positioned between the means for capturing the biomolecule and at least a portion of the inner surface of the reservoir, and an aperture defined in the inner surface of the reservoir. Some embodiments of the method include combining the sample with a solid phase that is adapted to capture the biomolecule, removing the insoluble matter from the sample, and removing the biomolecule from the solid phase.

US Patent:

62185314, Apr 17, 2001

Filed:

Dec 20, 1999

Appl. No.:

9/445944

Inventors:

Steven J. Ekenberg - Mount Horeb WI

Assignee:

Promega Corporation - Madison WI

International Classification:

C07H 2104
C12Q 168
C12P 1934
B01D 1508

US Classification:

536 2541

Abstract:

The present invention provides a method for isolating RNA from a biological material comprising RNA and contaminants, wherein: the biological material is disrupted in the presence of a chaotropic agent, the resulting lysate is diluted to precipitate out contaminants, and the precipitate is removed from the lysate. RNA is preferably isolated from the resulting cleared lysate, using a silica matrix to bind and then release RNA bound thereto under particular conditions. The present invention also provides a method for isolating RNA from a solution comprising RNA and DNA, wherein: the RNA and DNA are bound to a silica matrix in the presence of at least one binding enhancer, the DNA is digested with DNase, and the RNA fluted therefrom.