Steven W Metzger

US Patent:

7341841, Mar 11, 2008

Filed:

Dec 16, 2005

Appl. No.:

11/303803

Inventors:

Steven W. Metzger - Westminster CO, US
David C. Howson - Denver CO, US
David A. Goldberg - Boulder CO, US
Daniel A. Buttry - Laramie WY, US

Assignee:

Accelr8 Technology Corporation - Denver CO

International Classification:

G01N 33/53

US Classification:

435 72

Abstract:

A method for the detection of microorganisms in a sample comprising contacting said sample with a biosensor concentration module, allowing microorganisms to grow for a first period of time and detecting growth of discrete microorganisms as an indication of the presence of said microorganisms.

US Patent:

7501157, Mar 10, 2009

Filed:

Jan 13, 2005

Appl. No.:

11/035849

Inventors:

Guoqiang Mao - Smyrna GA, US
Steven W. Metzger - Westminster CO, US
Michael J. Lochhead - Boulder CO, US
David A. Goldberg - Boulder CO, US
Charles H. Greef - Louisville CO, US
David W. Grainger - Fort Collins CO, US

Assignee:

Accelr8 Technology Corporation - Denver CO

International Classification:

B05D 3/02

US Classification:

427384, 4273855, 4273881, 4273897, 4273935

Abstract:

Compositions and methods of preparing functional thin films or surface coatings with low non-specific binding are described. The thin films contain specified functional groups and non-specific binding repellant components. The thin films are either covalently bound to or passively adsorbed to various solid substrates. The specified functional group provides specified activity for the thin film modified solid surfaces and non-specific binding repellant components significantly reduce the non-specific binding to the thin film modified solid surfaces. Non-specific binding repellant components do not affect specified functional group's activity in the thin films. In these methods, specified functional groups are anchored to the solid substrates through a spacer. Surface coatings are also described having both non-specific protein binding properties combined with functional groups for specific binding activity thereby providing surface coating that specifically recognize target proteins but limit binding to non-specific protein.

US Patent:

7687239, Mar 30, 2010

Filed:

Jul 8, 2004

Appl. No.:

10/888828

Inventors:

David A. Goldberg - Boulder CO, US
David C. Howson - Denver CO, US
Steven W. Metzger - Westminster CO, US
Daniel A. Buttry - Laramie WY, US
Steven Scott Saavedra - Tucson AZ, US

Assignee:

Accelrs Technology Corporation - Denver CO

International Classification:

G01N 33/53

US Classification:

435 72

Abstract:

The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

US Patent:

8460887, Jun 11, 2013

Filed:

Feb 8, 2010

Appl. No.:

12/702210

Inventors:

David A. Goldberg - Boulder CO, US
David C. Howson - Denver CO, US
Steven W. Metzger - Westminster CO, US
Daniel A. Buttry - Laramie WY, US
Steven Scott Saavedra - Tucson AR, US

Assignee:

Accelerate Diagnostics, Inc. - Tucson AZ

International Classification:

G01N 33/53

US Classification:

435 72

Abstract:

The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

US Patent:

20030153023, Aug 14, 2003

Filed:

Feb 25, 2002

Appl. No.:

10/084632

Inventors:

Timothy Starzl - Boulder CO, US
Scott Clark - Denver CO, US
Mary Vellequette - Louisville CO, US
H. Hanlin - Louisville CO, US
Steven Metzger - Fort Collins CO, US

International Classification:

G01N033/53
G01N033/537
G01N033/543
G06F019/00
G06K009/00

US Classification:

435/007920, 382/128000, 702/019000

Abstract:

This invention is directed to an optically-based method and system for analyte detection using solid phase immobilization, specific analyte labels adapted for signal generation and corresponding processes for the utilization thereof. The enumeration detection method disclosed herein narrows the area for signal observation, thus, improving detectable signal to background ratio. The system is comprised of a platform/support for immobilizing a sample stage having a labeled sample (analyte complex) bound thereto, a radiation source, an optical apparatus for generating and directing radiation at said sample and a control that obtains data and then conducts analyses using digital image data. Upon engagement of the system, the sample generates a signal capable of differentiation from background signal, both of which are collected and imaged with a signal detector that generated a sample image to a data processing apparatus. This apparatus receives signal measurements and, in turn, enumerates individual binding events. Generated signal may be increased via selected mass enhancement. The invention, enumeration assay methodology detecting individual binding events, may be used, for example, in analyses to detect analyte or confirm results in both research, commercial and point of care applications.

US Patent:

20070298513, Dec 27, 2007

Filed:

Oct 23, 2006

Appl. No.:

11/552111

Inventors:

Timothy Starzl - Boulder CO, US
Scott Clark - Denver CO, US
Mary Vellequette - Louisville CO, US
H. Hanlin - Louisville CO, US
Steven Metzger - Fort Collins CO, US

Assignee:

Accelr8 Technology Corporation - Denver CO

International Classification:

G01N 21/01

US Classification:

436164000

Abstract:

This invention is directed to an optically-based method and system for analyte detection using solid phase immobilization, specific analyte labels adapted for signal generation and corresponding processes for the utilization thereof. The enumeration detection method disclosed herein narrows the area for signal observation, thus, improving detectable signal to background ratio. The system is comprised of a platform/support for immobilizing a sample stage having a labeled sample (analyte complex) bound thereto, a radiation source, an optical apparatus for generating and directing radiation at said sample and a control that obtains data and then conducts analyses using digital image data. Upon engagement of the system, the sample generates a signal capable of differentiation from background signal, both of which are collected and imaged with a signal detector that generated a sample image to a data processing apparatus. This apparatus receives signal measurements and, in turn, enumerates individual binding events. Generated signal may be increased via selected mass enhancement. The invention, enumeration assay methodology detecting individual binding events, may be used, for example, in analyses to detect analyte or confirm results in both research, commercial and point of care applications.

US Patent:

20120077206, Mar 29, 2012

Filed:

Dec 5, 2011

Appl. No.:

13/311100

Inventors:

Steven W. Metzger - Westminster CO, US
David C. Howson - Denver CO, US
David A. Goldberg - Boulder CO, US
Daniel A. Buttry - Laramie WY, US

Assignee:

Accelr8 Technology Corporation - Denver CO

International Classification:

C12Q 1/18
G01N 33/569

US Classification:

435 71, 435 32

Abstract:

A method for the detection of microorganisms in a sample comprising contacting said sample with a biosensor concentration module, allowing microorganisms to grow for a first period of time and detecting growth of discrete microorganisms as an indication of the presence of said microorganisms.

US Patent:

20140038171, Feb 6, 2014

Filed:

Mar 7, 2012

Appl. No.:

14/004145

Inventors:

Steven W. Metzger - Tucson AZ, US
Kenneth Robert Hance - Tucson AZ, US

Assignee:

Accelerate Technology Corporation - Denver CO

International Classification:

C12N 13/00

US Classification:

435 5, 4351739, 435 34

Abstract:

Methods and systems for purifying cells and/or viruses are provided. The sample is added to a well disposed in a medium. A potential is applied across the medium to cause the contaminants to enter one or more walls of the well, and retain the cells and/or viruses in the well. The cells and/or viruses can be removed from the well, and optionally adhered or fixed to a surface, or detected. In one embodiment, the cells and/or viruses may be retained in the well by embedding in the medium. The medium including the embedded cells and/or viruses may be excised or otherwise removed and transferred to a glass slide or other solid surface.