Steven J Norberg

US Patent:

20220282242, Sep 8, 2022

Filed:

Apr 12, 2022

Appl. No.:

17/719276

Inventors:

- Essex, GB
Kevin L. Gunderson - San Diego CA, US
Fan Zhang - San Diego CA, US
Jason Richard Betley - Essex, GB
Niall Anthony Gormley - Essex, GB
Wouter Meuleman - San Diego CA, US
Jacqueline Weir - Essex, GB
Avgousta Ioannou - Essex, GB
Gareth Jenkins - Essex, GB
Rosamond Jackson - Essex, GB
Natalie Morrell - Essex, GB
Dmitry K. Pokholok - San Diego CA, US
Steven J. Norberg - San Diego CA, US
Molly He - San Diego CA, US
Amirali Kia - San Diego CA, US
Igor Goryshin - Madison WI, US
Rigo Pantoja - San Diego CA, US

International Classification:

C12N 15/10
C12Q 1/6806
C12Q 1/6874
C12Q 1/6876

Abstract:

Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

US Patent:

20200165650, May 28, 2020

Filed:

Oct 21, 2019

Appl. No.:

16/659475

Inventors:

- San Diego CA, US
Jeffrey G. Mandell - La Jolla CA, US
Alex Aravanis - San Mateo CA, US
Steven Norberg - San Diego CA, US
Dmitry K. Pokholok - San Marcos CA, US
Frank J. Steemers - Encinitas CA, US
Farnaz Absalan - San Francisco CA, US
Leila Bazargan - Palo Alto CA, US

International Classification:

C12P 19/34
C12Q 1/683
C12Q 1/6869
C12Q 1/6816
C12N 15/10

Abstract:

A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

US Patent:

20190352591, Nov 21, 2019

Filed:

Apr 15, 2019

Appl. No.:

16/384741

Inventors:

- San Diego CA, US
Ramesh Ramji - San Diego CA, US
Steven Norberg - La Mesa CA, US
Lena Christiansen - San Diego CA, US
Dmitry K. Pokholok - San Diego CA, US
Fan Zhang - San Diego CA, US

International Classification:

C12M 1/12
C12N 5/16
C12N 5/00
A61K 47/58
A61K 47/69

Abstract:

Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

US Patent:

20190048332, Feb 14, 2019

Filed:

Oct 29, 2018

Appl. No.:

16/173202

Inventors:

- Essex, GB
Kevin L. Gunderson - San Diego CA, US
Fan Zhang - San Diego CA, US
Jason Richard Betley - Essex, GB
Niall Anthony Gormley - Essex, GB
Wouter Meuleman - San Diego CA, US
Jacqueline Weir - Essex, GB
Avgousta Ioannou - Essex, GB
Gareth Jenkins - Essex, GB
Rosamond Jackson - Essex, GB
Natalie Morrell - Essex, GB
Dmitry K. Pokholok - San Diego CA, US
Steven J. Norberg - San Diego CA, US
Molly He - San Diego CA, US
Amirali Kia - San Diego CA, US
Igor Goryshin - Madison WI, US
Rigo Pantoja - San Diego CA, US

International Classification:

C12N 15/10
C12Q 1/6876
C12Q 1/6806
C12Q 1/6874

Abstract:

Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

US Patent:

20190040382, Feb 7, 2019

Filed:

Oct 16, 2015

Appl. No.:

15/519482

Inventors:

Frank J. STEEMERS - San Diego CA, US
Kevin L. GUNDERSON - San Diego CA, US
Fan ZHANG - San Diego CA, US
Jason Richard BETLEY - Essex, GB
Niall Anthony GORMLEY - Essex, GB
Wouter MEULEMAN - San Diego CA, US
Jacqueline WEIR - Essex, GB
Avgousta IOANNOU - Essex, GB
Gareth JENKINS - Essex, GB
Rosamond JACKSON - Essex, GB
Natalie MORRELL - Essex, GB
Dmitry K. POKHOLOK - San Diego CA, US
Steven J. NORBERG - San Diego CA, US
Molly HE - San Diego CA, US
Amirali KIA - San Diego CA, US
Igor GORYSHIN - Madison WI, US
Rigo PANTOJA - San Diego CA, US
- San Diego CA, US

Assignee:

ILLUMINA CAMBRIDGE LIMITED - Essex

International Classification:

C12N 15/10
C12Q 1/68

Abstract:

Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

US Patent:

20180355348, Dec 13, 2018

Filed:

Jun 5, 2018

Appl. No.:

16/000505

Inventors:

- Portland OR, US
- San Diego CA, US
Frank J. Steemers - San Diego CA, US
Dmitry K. Pokholok - San Diego CA, US
Steven Norberg - San Diego CA, US

Assignee:

OREGON HEALTH & SCIENCE UNIVERSITY - Portland OR
ILLUMINA, INC. - San Diego CA

International Classification:

C12N 15/10

Abstract:

Provided herein are methods for preparing sequencing libraries for determining the methylation status of nucleic acids from a plurality of single cells. The present methods combine split-and-pool combinatorial indexing and bisulfite treatment techniques to characterize the methylation profiles of large numbers of single cells quickly, accurately and inexpensively.

US Patent:

20160017396, Jan 21, 2016

Filed:

Jul 20, 2015

Appl. No.:

14/804068

Inventors:

- San Diego CA, US
Jeffrey G. Mandell - La Jolla CA, US
Alex Aravanis - San Mateo CA, US
Steven Norberg - San Diego CA, US
Dmitry K. Pokholok - San Marcos CA, US
Frank J. Steemers - Encinitas CA, US
Farnaz Absalan - San Francisco CA, US
Leila Bazargan - Palo Alto CA, US

Assignee:

ILLUMINA, INC. - San Diego CA

International Classification:

C12P 19/34

Abstract:

A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.