Steven C Phelps

US Patent:

20140066326, Mar 6, 2014

Filed:

Aug 30, 2013

Appl. No.:

14/014607

Inventors:

Lizhong DAI - San Diego CA, US
Steven PHELPS - La Jolla CA, US

Assignee:

GEN-PROBE INCORPORATED - San Diego CA

International Classification:

C12Q 1/68
C12Q 1/70

US Classification:

506 9, 435 611, 435 5

Abstract:

Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

US Patent:

20210403982, Dec 30, 2021

Filed:

May 24, 2021

Appl. No.:

17/328312

Inventors:

- San Diego CA, US
Lizhong DAI - San Diego CA, US
Steven PHELPS - La Jolla CA, US

International Classification:

C12Q 1/6806
C12Q 1/6848
C12Q 1/70
C12P 19/34
C12Q 1/68
C12Q 1/6865
C12Q 1/6825

Abstract:

Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

US Patent:

20210270708, Sep 2, 2021

Filed:

May 19, 2021

Appl. No.:

17/303043

Inventors:

- San Diego CA, US
Michael Dai Wang - San Diego CA, US
Umberto Ulmanella - San Diego CA, US
James Michael Osmus - San Diego CA, US
Stephen Wayne Clark - San Diego CA, US
Johanna Lynn Whitacre - San Diego CA, US
Steven Scott Phelps - San Diego CA, US
Michelle L. Alvarez - San Diego CA, US
Michael Adalbert Niziolek - San Diego CA, US
Debra Sue Bryan - San Diego CA, US
Joshua Augustin Darland - San Diego CA, US

Assignee:

Illumina, Inc. - San Diego CA

International Classification:

G01N 1/38
B01L 3/00
G01N 35/10
C12Q 1/6874
B01F 13/10
B01F 15/02
B01F 5/00
B01F 5/06
B01F 3/08
G01N 1/14
B01F 11/00
B01F 15/00

Abstract:

An analysis instrument may perform analytical operations on an analyte that is combined with multiple reagents prior to being introduced into a flow cell. The instrument may include a nozzle sipper that aspirates reagents from a recipient, along with an analyte. The reagents may be directed to a volume and may be repeatedly moved into and out of the volume by cycling of a pump. The reagents may be ejected into a destination recipient with the nozzle sipper promoting vorticity in the recipient to enhance mixing. The repeated aspiration and ejection through the nozzle sipper effectively mixes the reagents and the template in an automated or semi-automated fashion.

US Patent:

20210018527, Jan 21, 2021

Filed:

Oct 2, 2020

Appl. No.:

17/062288

Inventors:

- San Diego CA, US
Steven S. Phelps - San Diego CA, US
Bradley Kent Drews - Poway CA, US
Michael A. Niziolek - San Diego CA, US
Joshua A. Darland - Pleasanton CA, US
Umberto Ulmanella - San Diego CA, US
Michael Dai Wang - San Diego CA, US
Michelle L. Alvarez - Encinitas CA, US
Stephen Wayne Clark - San Diego CA, US

International Classification:

G01N 35/10
G01N 35/00
B01L 3/00
B01F 15/02
B01F 5/00
B01F 5/06
B01F 3/08
B01F 13/10
C12Q 1/6874
B01F 15/00
B01L 3/02
B01F 11/00

Abstract:

A method includes, under control of control circuitry implementing a mixing protocol, aspirating reagents from multiple different reagent reservoirs into a cache channel. Designated amounts of the reagents are automatically aspirated from the corresponding reagent reservoirs by corresponding sippers based on the mixing protocol implemented by the control circuitry. The method also includes discharging the reagents from the cache channel into a mixing reservoir, and mixing the reagents within the mixing reservoir to form a reagent mixture.

US Patent:

20200230598, Jul 23, 2020

Filed:

Mar 23, 2020

Appl. No.:

16/827391

Inventors:

- San Diego CA, US
Michael Dai Wang - San Diego CA, US
Umberto Ulmanella - San Diego CA, US
Stephen Wayne Clark - San Diego CA, US
Johanna Lynn Whitacre - San Diego CA, US
Steven Scott Phelps - San Diego CA, US
Michelle L. Alvarez - San Diego CA, US
Michael Adalbert Niziolek - San Diego CA, US
Debra Sue Bryan - San Diego CA, US
Joshua Augustin Darland - San Diego CA, US

International Classification:

B01L 3/00
G01N 35/10
B01F 15/02
B01F 13/00
B01F 11/00

Abstract:

An analysis system may perform operations on an analyte that may be combined with multiple regents prior to being introduced into a flow cell. The instrument may include a volume into which the reagents to be combined with the analyte are aspirated one-by-one. The volume may be formed as a serpentine channel in a valve manifold associated with sippers for aspirating the reagents. The reagents may then be mixed by cycling a pump to move the reagents within the mixing volume or channel. For this, the reagents may be aspirated from a recipient into the volume or channel, ejected back into the recipient, and this process may be performed repeatedly to enhance mixing

US Patent:

20190185911, Jun 20, 2019

Filed:

Jan 7, 2019

Appl. No.:

16/241778

Inventors:

- San Diego CA, US
Lyle J. ARNOLD - Poway CA, US
Lizhong DAI - San Diego CA, US
Steven PHELPS - La Jolla CA, US

International Classification:

C12Q 1/6806
C12Q 1/6865
C12Q 1/6825
C12Q 1/6848
C12P 19/34
C12Q 1/70
C12Q 1/68

Abstract:

Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

US Patent:

20180185842, Jul 5, 2018

Filed:

Dec 13, 2017

Appl. No.:

15/841102

Inventors:

- San Diego CA, US
Michael Dai Wang - San Diego CA, US
Umberto Ulmanella - San Diego CA, US
Stephen Wayne Clark - San Diego CA, US
Johanna Lynn Whitacre - San Diego CA, US
Steven Scott Phelps - San Diego CA, US
Michelle L. Alvarez - San Diego CA, US
Michael Adalbert Niziolek - San Diego CA, US
Debra Sue Bryan - San Diego CA, US
Joshua Augustin Darland - San Diego CA, US

International Classification:

B01L 3/00

Abstract:

An analysis system may perform operations on an analyte that may be combined with multiple regents prior to being introduced into a flow cell. The instrument may include a volume into which the reagents to be combined with the analyte are aspirated one-by-one. The volume may be formed as a serpentine channel in a valve manifold associated with sippers for aspirating the reagents. The reagents may then be mixed by cycling a pump to move the reagents within the mixing volume or channel. For this, the reagents may be aspirated from a recipient into the volume or channel, ejected back into the recipient, and this process may be performed repeatedly to enhance mixing

US Patent:

20180188141, Jul 5, 2018

Filed:

Dec 13, 2017

Appl. No.:

15/841098

Inventors:

- San Diego CA, US
Michael Dai Wang - San Diego CA, US
Umberto Ulmanella - San Diego CA, US
James Michael Osmus - San Diego CA, US
Stephen Wayne Clark - San Diego CA, US
Johanna Lynn Whitacre - San Diego CA, US
Steven Scott Phelps - San Diego CA, US
Michelle L. Alvarez - San Diego CA, US
Michael Adalbert Niziolek - San Diego CA, US
Debra Sue Bryan - San Diego CA, US
Joshua Augustin Darland - San Diego CA, US

International Classification:

G01N 1/38
G01N 1/14
B01L 3/00
G01N 35/10
C12Q 1/6874
B01F 13/10
B01F 15/02
B01F 5/00
B01F 5/06
B01F 3/08

Abstract:

An analysis instrument may perform analytical operations on an analyte that is combined with multiple reagents prior to being introduced into a flow cell. The instrument may include a nozzle sipper that aspirates reagents from a recipient, along with an analyte. The reagents may be directed to a volume and may be repeatedly moved into and out of the volume by cycling of a pump. The reagents may be ejected into a destination recipient with the nozzle sipper promoting vorticity in the recipient to enhance mixing. The repeated aspiration and ejection through the nozzle sipper effectively mixes the reagents and the template in an automated or semi-automated fashion.