Steven M Riddle

US Patent:

8409814, Apr 2, 2013

Filed:

Sep 1, 2010

Appl. No.:

12/874140

Inventors:

Kurt Vogel - Madison WI, US
Rhonda Newman - Eugene OR, US
Steven Riddle - Madison WI, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

G01N 33/53
G01N 33/00
C07K 1/00
C07K 14/00
C07K 16/00
C07K 17/00

US Classification:

435 71, 436103, 530400, 530402, 530408, 530409, 530410

Abstract:

The present invention relates to biosensors. In some embodiments, the biosensors are modified ligand binding molecules. In some embodiments, the modified ligand binding molecule is a phosphate binding protein (PBP). In some embodiments, the modified ligand binding molecules are labeled to be capable of RET, e. g. , comprising a donor and acceptor moiety. In some embodiments of the invention, there is a detectable change in RET (e. g. , FRET) when the modified ligand binding molecule binds and/or releases the ligand (e. g. , phosphate). The invention also provides related methods, reactions and assays.

US Patent:

8642256, Feb 4, 2014

Filed:

Jul 23, 2009

Appl. No.:

12/508513

Inventors:

Kurt Vogel - Madison WI, US
Steven Riddle - Madison WI, US
Robert Horton - Madison WI, US
Matthew Robers - Madison WI, US
Gregory Michaud - Stow MA, US
Thomas Machleidt - Madison WI, US
Kevin Vedvik - Sun Prairie WI, US
Kristin Huwiler - Madison WI, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12Q 1/00

US Classification:

435 4

Abstract:

Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing molecular interactions (e. g. , deubiquination, ubiquination and kinase activity), e. g. , using resonance energy transfer (RET) are provided.

US Patent:

20070264678, Nov 15, 2007

Filed:

Oct 30, 2006

Appl. No.:

11/554553

Inventors:

Kurt VOGEL - Madison WI, US
Steven RIDDLE - Madison WI, US
Robert HORTON - Madison WI, US
Matthew ROBERS - Middleton WI, US
Gregory MICHAUD - Clinton CT, US
Thomas MACHLEIDT - Madison WI, US
Kevin VEDVIK - Sun Prairie WI, US
Kristin HUWILER - Madison WI, US

Assignee:

INVITROGEN CORPORATION - Carlsbad CA

International Classification:

C12Q 1/48

US Classification:

435015000

Abstract:

Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing molecular interactions (e.g., deubiquination, ubiquination and kinase activity), e.g., using resonance energy transfer (RET) are provided.

US Patent:

20080138836, Jun 12, 2008

Filed:

Jul 20, 2007

Appl. No.:

11/781124

Inventors:

Gregory Michaud - Stow MA, US
Kurt Vogel - Madison WI, US
Matthew Robers - Madison WI, US
Robert Horton - Madison WI, US
Steven Riddle - Madison WI, US

Assignee:

INVITROGEN CORPORATION - Carlsbad CA

International Classification:

G01N 33/573
G01N 33/53

US Classification:

435 74, 435 71

Abstract:

Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing molecular interactions (e.g., deubiquination, ubiquination and kinase activity) using resonance energy transfer (RET) are provided.

US Patent:

20080213811, Sep 4, 2008

Filed:

Aug 10, 2007

Appl. No.:

11/837180

Inventors:

Kurt Vogel - Madison WI, US
Rhonda Newman - Eugene OR, US
Steven Riddle - Madison WI, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12Q 1/44
G01N 33/48
C12Q 1/48

US Classification:

435 15, 436 63, 435 19

Abstract:

The present invention relates to biosensors. In some embodiments, the biosensors are modified ligand binding molecules. In some embodiments, the modified ligand binding molecule is a phosphate binding protein (PBP). In some embodiments, the modified ligand binding molecules are labeled to be capable of RET, e.g., comprising a donor and acceptor moiety. In some embodiments of the invention, there is a detectable change in RET (e.g., FRET) when the modified ligand binding molecule binds and/or releases the ligand (e.g., phosphate). The invention also provides related methods, reactions and assays.