Steven R Schwarz

US Patent:

56655762, Sep 9, 1997

Filed:

Jun 7, 1995

Appl. No.:

8/476286

Inventors:

Paul M. Cino - Bound Brook NJ
Steven R. Schwarz - Milltown NJ
Dana L. Cazzulino - New York NY

Assignee:

E. R. Squibb & Sons, Inc. - Princeton NJ

International Classification:

C12P 100
C12P 722
C12N 500

US Classification:

435156

Abstract:

A method for the production of taxanes such as taxol. The method includes inducing formation of callus cells by contacting an explant tissue with a liquid medium without complete submergence of the tissue in the medium. The callus cells formed are employed in a liquid suspension cell culture to produce one or more taxanes.

US Patent:

55277020, Jun 18, 1996

Filed:

Feb 23, 1994

Appl. No.:

8/202718

Inventors:

Paul M. Cino - Bound Brook NJ
Steven R. Schwarz - Milltown NJ
Dana L. Cazzulino - New York NY

Assignee:

E. R. Squibb & Sons, Inc. - Princeton NJ

International Classification:

C12N 500
C12P 1704

US Classification:

43524048

Abstract:

A method for the induction of callus cells capable of producing taxanes from explant tissue by partially submerging the explant tissue in a liquid culture medium on a membrane raft, resulting in callus formation. The induction of callus cells by this method provides direct transfer of the cells formed to a liquid medium for a suspension cell culture preparation of taxanes. Thus, there is no need of a separate growth or proliferation step in the method of this invention. Furthermore, callus cells can be produced by the method; and also disclosed is a method for the use of the callus cells so produced in a suspension cell culture preparation for taxanes.