Stewart A Craig

US Patent:

6797514, Sep 28, 2004

Filed:

Sep 20, 2001

Appl. No.:

09/960264

Inventors:

Ronald Berenson - Mercer Island WA
Che Law - Shoreline WA
Mark Bonyhadi - Issaquah WA
Narinder Saund - Seattle WA
Stewart Craig - Issaquah WA
Alan Hardwick - Seattle WA
Dale Kalamasz - Redmond WA
David McMillen - Seattle WA

Assignee:

Xcyte Therapies, Inc. - Seattle WA

International Classification:

C12N 500

US Classification:

435325, 4353723, 436526

Abstract:

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

US Patent:

6867041, Mar 15, 2005

Filed:

Apr 26, 2002

Appl. No.:

10/133236

Inventors:

Ronald Berenson - Mercer Island WA, US
Che Law - Shoreline WA, US
Mark Bonyhadi - Issaquah WA, US
Narinder Saund - Seattle WA, US
Stewart Craig - Issaquah WA, US
Alan Hardwick - Seattle WA, US
Dale Kalamasz - Redmond WA, US
David McMillen - Seattle WA, US

Assignee:

XCYTE Therapies, Inc. - Seattle WA

International Classification:

C12N005/00

US Classification:

435377, 435325, 435326, 435375, 436526

Abstract:

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and/or stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

US Patent:

6905874, Jun 14, 2005

Filed:

Feb 26, 2001

Appl. No.:

09/794230

Inventors:

Ronald Berenson - Mercer Island WA, US
Che Law - Shoreline WA, US
Mark Bonyhadi - Issaquah WA, US
Narinder Saund - Seattle WA, US
Stewart Craig - Issaquah WA, US
Alan Hardwick - Seattle WA, US
Dale Kalamasz - Redmond WA, US
David McMillen - Seattle WA, US

Assignee:

XCYTE Therapies, Inc. - Seattle WA

International Classification:

C12N005/00

US Classification:

435375, 435325, 4353723, 436526

Abstract:

The present invention relates generally to methods for stimulating cells, and more particularly, to a novel method to concentrate and stimulate cells that maximizes stimulation and/or proliferation of such cells. In the various embodiments, cells are stimulated and concentrated with a surface yielding enhanced proliferation, cell signal transduction, and/or cell surface moiety aggregation. In certain aspects methods for stimulating a population of cells such as T-cells, by simultaneous concentration and cell surface moiety ligation are provided by contacting the population of cells with a surface, that has attached thereto one or more agents that ligate a cell surface moiety and applying a force that predominantly drives cell concentration and cell surface moiety ligation, thereby inducing cell stimulation, cell surface moiety aggregation, and/or receptor signaling enhancement. Also provided are methods for producing phenotypically tailored cells, including T-cells for the use in diagnostics, drug discovery, and the treatment of a variety of indications, including cancer, viral infection, and immune related disorders. Compositions of cells having specific phenotypic properties produced by these processes are further provided.

US Patent:

7541184, Jun 2, 2009

Filed:

Jan 20, 2004

Appl. No.:

10/762210

Inventors:

Ronald J. Berenson - Mercer Island WA, US
Che Law - Shoreline WA, US
Mark Bonyhadi - Issaquah WA, US
Narinder Saund - Seattle WA, US
Stewart Craig - Issaquah WA, US
Alan Hardwick - Seattle WA, US
Dale Kalamasz - Redmond WA, US
David McMillen - Seattle WA, US
Harjinder Singh Chana - Issaquah WA, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 5/00

US Classification:

435375, 435325, 4353723

Abstract:

The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

US Patent:

7572631, Aug 11, 2009

Filed:

Mar 2, 2005

Appl. No.:

11/070841

Inventors:

Ronald J Berenson - Mercer Island WA, US
Che Law - Shoreline WA, US
Mark Bonyhadi - Issaquah WA, US
Zahbea Saund - Seattle WA, US
Stewart Craig - Issaquah WA, US
Alan Hardwick - Seattle WA, US
Dale Kalamasz - Redmond WA, US
David McMillen - Seattle WA, US
Harjinder Singh Chana - Issaquah WA, US

Assignee:

Invitrogen Corporation - Carlsbad CA

International Classification:

C12N 5/00

US Classification:

435375, 435325, 4353723

Abstract:

The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

US Patent:

20030082806, May 1, 2003

Filed:

Apr 29, 2002

Appl. No.:

10/136024

Inventors:

Ronald Berenson - Mercer Island WA, US
Mark Bonyhadi - Issaquah WA, US
Stewart Craig - Issaquah WA, US
Dale Kalamasz - Redmond WA, US
Tatsue Monji - Seattle WA, US

Assignee:

XCYTE Therapies, Inc. - Seattle WA

International Classification:

A61K048/00
C12N005/08

US Classification:

435/372000, 424/093210

Abstract:

The present invention relates to methods for maturing antigen-presenting cells, and more particularly, to methods for maturing dendritic cells. Methods for generating mature and/or maturing antigen-presenting cells in vitro and in vivo are disclosed. The present invention also relates to compositions of cells, including mature antigen-presenting cells and/or activated T cells and their use in generating immune responses in vivo, and inhibiting the development of or preventing infectious diseases and cancers.

US Patent:

20030119185, Jun 26, 2003

Filed:

Jun 28, 2002

Appl. No.:

10/187467

Inventors:

Ronald Berenson - Mercer Island WA, US
Che Law - Shoreline WA, US
Mark Bonyhadi - Issaquah WA, US
Narinder Saund - Seattle WA, US
Stewart Craig - Issaquah WA, US
Alan Hardwick - Seattle WA, US
Dale Kalamasz - Redmond WA, US
David McMillen - Seattle WA, US
Harjinder Chana - Issaquah WA, US

Assignee:

XCYTE Therapies, Inc. - Seattle WA

International Classification:

C12N005/08

US Classification:

435/372000

Abstract:

The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.

US Patent:

20030235908, Dec 25, 2003

Filed:

Jan 22, 2003

Appl. No.:

10/350305

Inventors:

Ronald Berenson - Mercer Island WA, US
Che Law - Shoreline WA, US
Mark Bonyhadi - Issaquah WA, US
Narinder Saund - Seattle WA, US
Stewart Craig - Issaquah WA, US
Alan Hardwick - Seattle WA, US
Dale Kalamasz - Redmond WA, US
David McMillen - Seattle WA, US
Harjinder Chana - Issaquah WA, US

Assignee:

XCYTE Therapies, Inc. - Seattle WA

International Classification:

C12N005/08

US Classification:

435/372000

Abstract:

The present invention relates generally to methods for activating and expanding cells, and more particularly, to a novel method to activate and/or stimulate cells that maximizes the expansion of such cells to achieve dramatically high densities. In the various embodiments, cells are activated and expanded to very high densities in a short period of time. In certain embodiments, cells are activated and expanded to very high numbers of cells in a short period of time. Compositions of cells activated and expanded by the methods herein are further provided.