Stuart M Levy

US Patent:

6346391, Feb 12, 2002

Filed:

Jul 22, 1999

Appl. No.:

09/358948

Inventors:

Margaret Oethinger - Bad Oeynhausen, DE
Stuart B. Levy - Boston MA

Assignee:

Trustees of Tufts College - Medford MA

International Classification:

C12Q 118

US Classification:

435 32, 435 29, 435 4, 435 15, 435 16, 435 23, 435 24

Abstract:

The instant methods and compositions represent an advance in controlling drug resistance in microbes. AcrAB-like efflux pumps have been found to control resistance to drugs, even in highly resistant microbes. Accordingly, methods of treating infection, methods of screening for inhibitors of AcrAB-like efflux pumps, and methods of enhancing antimicrobial activity of drugs are provided. Pharmaceutical composition comprising an inhibitor of an AcrAB-like efflux pump and an antimicrobial agent are also provided.

US Patent:

6391545, May 21, 2002

Filed:

Jul 17, 1998

Appl. No.:

09/118445

Inventors:

Stuart B. Levy - Boston MA

Assignee:

Trustees of Tufts College - Medford MA

International Classification:

C12Q 168

US Classification:

435 6, 536 241

Abstract:

An isolated and cloned region of a bacterial chromosome containing a multiple antibiotic resistance operon is disclosed. A description of the structure and function of the operon is provided as are assorted recombinant DNA constructs involving the operon or fragments thereof. The diagnostic, therapeutic and experimental uses of these constructs are also disclosed. Methods of evaluating the antibiotic effectiveness of compositions are disclosed and methods of treatment employing effective compositions are provided.

US Patent:

6448006, Sep 10, 2002

Filed:

Feb 29, 2000

Appl. No.:

09/515705

Inventors:

Stuart B. Levy - Boston MA

Assignee:

Trustees of Tufts College - Boston MA

International Classification:

C12Q 168

US Classification:

435 6, 435 4, 435 71, 435 691, 435 9131, 536 231, 536 241, 536 245

Abstract:

The invention relates to methods and compositions for manipulating bacterial resistance to non-antibiotic antibacterial compositions, disinfectants and organic solvents. The invention provides methods for rendering bacterial cells susceptible to non-antibiotic antibacterial compositions. Also provided are methods to reduce the selection of bacterial mutants having an multiple antibiotic resistance phenotype by non-antibiotic antibacterial compositions. The invention also provides methods for testing the ability of non-antibiotic antibacterial compositions to select for or induce a multiple antibiotic resistance phenotype in bacteria. Also provided are methods for increasing or decreasing bacterial tolerance to organic solvents by increasing or decreasing the activity of bacterial organic solvent efflux pumps. Compositions useful in the foregoing methods are also provided.

US Patent:

6485973, Nov 26, 2002

Filed:

Jun 16, 2000

Appl. No.:

09/596390

Inventors:

Stuart B. Levy - Boston MA
Eric Von Hofe - Wellesley MA

Assignee:

Hybridon, Inc. - Cambridge MA
Trustees of Tufts College - Medford MA

International Classification:

C07H 2104

US Classification:

435375, 435 6, 435455, 536 231, 536 241, 536 245

Abstract:

Disclosed are synthetic oligonucleotides complementary to a transcript of the marORAB operon which inhibit expression of a gene in the operon. Also disclosed are methods of reducing bacterial resistance to antibiotics, and pharmaceutical formulations containing marORAB-specific oligonucleotides of the invention.

US Patent:

6677133, Jan 13, 2004

Filed:

Sep 28, 2001

Appl. No.:

09/966835

Inventors:

Margaret Oethinger - Bad Oeynhausen, DE
Stuart B. Levy - Boston MA

Assignee:

Trustees of Tufts College - Medford MA

International Classification:

C12Q 118

US Classification:

435 32, 435 29, 435 4, 435 15, 435 16, 435 23, 435 24

Abstract:

The instant methods and compositions represent an advance in controlling drug resistance in microbes. AcrAB-like efflux pumps have been found to control resistance to drugs, even in highly resistant microbes. Accordingly, methods of treating infection, methods of screening for inhibitors of AcrAB-like efflux pumps, and methods of enhancing antimicrobial activity of drugs are provided. Pharmaceutical composition comprising an inhibitor of an AcrAB-like efflux pump and an antimicrobial agent are also provided.

US Patent:

6756365, Jun 29, 2004

Filed:

Feb 16, 2000

Appl. No.:

09/504697

Inventors:

Stuart B. Levy - Boston MA

Assignee:

Trustees of Tufts College - Medford MA

International Classification:

A61K 3165

US Classification:

514154

Abstract:

The present invention provides an improved methodology by which therapeutically to overcome resistance to tetracycline in living cells including bacteria, parasites, fungi, and rickettsiae. The methodology employs a blocking agent such as C5 ester derivatives, or 6-deoxy 13-(substituted mercapto) derivatives of tetracycline, in combination with other tetracycline-type antibiotics as a synergistic combination of compositions to be administered simultaneously, sequentially or concurrently. In another embodiment, certain novel compositions are provided which may be administered alone against, for example, a sensitive or resistant strain of gram positive bacteria such as and. The concomitantly administered compositions effectively overcome the tetracycline resistant mechanisms present such that the cell is effectively converted from a tetracycline-resistant state to a tetracycline-sensitive state.

US Patent:

6818634, Nov 16, 2004

Filed:

Mar 30, 2001

Appl. No.:

09/823884

Inventors:

Mark L. Nelson - Wellesley MA
Stuart B. Levy - Boston MA
Roger Frechette - Reading MA
Todd E. Bowser - Charlton MA
Mohamed Y. Ismail - Bedford MA

Assignee:

Paratek Pharmaceuticals, Inc. - Boston MA
Trustees of Tufts College - Medford MA

International Classification:

A61K 3165

US Classification:

514152, 548194

Abstract:

Substituted tetracycline compounds, methods of synthesis, and methods of use are discussed. Tetracyclines useful for treating tetracycline related disorders are also discussed. Intermediates useful for synthesizing other tetracycline compounds are also included.

US Patent:

6833365, Dec 21, 2004

Filed:

Jan 23, 2001

Appl. No.:

09/768189

Inventors:

Stuart B. Levy - Boston MA
Mark L. Nelson - Wellesley MA

Assignee:

Trustees of Tufts College - Medford MA

International Classification:

A61K 3165

US Classification:

514152

Abstract:

Methods and compositions for treating related disorders in a mammal are discussed. Several novel tetracycline compounds useful for treating related disorders are also included.