Sumita K Chakraborty

US Patent:

20140017718, Jan 16, 2014

Filed:

Sep 18, 2013

Appl. No.:

14/030886

Inventors:

Kenneth Alfano - Canton MI, US
Sumita Chakraborty - Ann Arbor MI, US

Assignee:

Blaze Medical Devices, LLC - Ann Arbor MI

International Classification:

G01N 33/49

US Classification:

435 29, 4352887

Abstract:

A test for erythrocyte membrane mechanical fragility, involving subjection of a sample comprising red blood cells to a mechanical stress capable of causing hemolysis, and measurement of the hemolysis present after particular extent(s) of the stress. Measurement of hemolysis can be without component separation, such as by cell-counting (optical or non-optical) or by spectral differentiation between cell-free and intracellular hemoglobin. Such tests can incorporate a disposable single-use component that contains an RBC sample during testing, and for certain embodiments the mechanical stress involved comprises (higher-energy) ultrasonic stress.

US Patent:

20130098163, Apr 25, 2013

Filed:

Dec 8, 2012

Appl. No.:

13/708980

Inventors:

Kenneth Alfano - Canton MI, US
Sumita Chakraborty - Ann Arbor MI, US

Assignee:

BLAZE MEDICAL DEVICES, LLC - Ann Arbor MI

International Classification:

G01B 17/00

US Classification:

73778

Abstract:

A device for testing erythrocyte membrane mechanical fragility that incorporates single-use disposable containers for holding cell samples, the device comprising: a stressor for subjecting a sample comprising red blood cells to a mechanical stress capable of causing hemolysis, wherein said sample remains within a disposable component during the subjecting; and a detector for direct or indirect measurement of said hemolysis present in said sample after particular extent(s) of said stress, whereby said measurement can occur while said sample remains within said component. In addition, the present disclosure specifically addresses such systems wherein said mechanical stress is ultrasonic stress.

US Patent:

20160305929, Oct 20, 2016

Filed:

Apr 13, 2016

Appl. No.:

15/098228

Inventors:

- Ann Arbor MI, US
Michael Tarasev - Pinckney MI, US
Sumita Chakraborty - Ann Arbor MI, US

International Classification:

G01N 33/49
G01N 3/24

Abstract:

A method using variable bead-oscillation-based stress parameters, including relative bead dimensionality or other structural features as well as stress duration or intensity or overall magnitude, in inducing different shear stresses in biological samples, for testing the effects of different combinations of various defined stress conditions.

US Patent:

20140315289, Oct 23, 2014

Filed:

Jun 30, 2014

Appl. No.:

14/320344

Inventors:

- Ann Arbor MI, US
Kenneth Alfano - Canton MI, US
Sumita Chakraborty - Ann Arbor MI, US

International Classification:

G01N 33/49

US Classification:

4352887

Abstract:

A disposable cartridge for use in red blood cell mechanical fragility profiling, comprising a stressing section with a lysing chamber in which the sample can receive multiple increments of mechanical stress to cause lysis, and a detection section with a transparent region that at least sometimes can have a thickness suitable to take optical readings that can directly or indirectly reflect a fraction/proportion of cells lysed after each increment of the stress, whereby said region can capture at least a representative portion of the sample that received each increment of stress. In some embodiments, the stressing section and the detection section are the same, and/or the transparent region is flexible or otherwise compressible, and/or the stress is ultrasonic. In some embodiments, the sections are separate, and/or the region is rigid or has a fixed thickness, and/or the stress may comprise cell-to-wall interaction and associated fluidic or viscous shear stress induced via concentric/coaxial objects.