Suzanne Cheng - Oakland CA, US Thomas W. Myers - Sunnyvale CA, US Nancy Patten - Oakland CA, US Nancy Schoenbrunner - Moraga CA, US Sim C. Truong - Union City CA, US
Assignee:
ROCHE MOLECULAR SYSTEMS, INC. - Pleasanton CA
International Classification:
C12N 15/11 G06F 19/12
US Classification:
435 912, 536 241, 703 11
Abstract:
The invention comprises suppressor oligonucleotides for reducing amplification of a non-target nucleic acid sequences; the method of designing and using such oligonucleotides, as well as kits and reaction mixtures.
Methods And Reagents For The Polymerase Chain Reaction Amplification Of Long Dna Sequences
Methods and reagents are provided for the amplification of DNA sequences longer than 10 kilobases by the polymerase chain reaction (PCR). The methods use compositions consisting of a primary thermostable DNA polymerase from Thermus thermophilus combined with a lesser amount of a secondary thermostable DNA polymerase possessing a 3'-to-5' exonuclease activity from Thermococcus litoralis, Pyrococcus species GB-D or Thermotoga maritima. The DNA polymerase compositions, when used with the disclosed reaction buffer, enable amplifications of DNA sequences up to at least 42. 2 kilobases in length.
Suppression Of Non-Specific Amplification With High-Homology Oligonucleotides
- Pleasanton CA, US Suzanne Cheng - Oakland CA, US Thomas W. Myers - Mountain View CA, US Nancy Patten - Oakland CA, US Nancy Schoenbrunner - Charlestown MA, US Sim C. Truong - Union City CA, US
International Classification:
C12Q 1/68 G06F 17/50 G06F 19/12
Abstract:
The invention comprises suppressor oligonucleotides for reducing amplification of a non-target nucleic acid sequences; the method of designing and using such oligonucleotides, as well as kits and reaction mixtures.
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