Terence E Flynn

US Patent:

20030198576, Oct 23, 2003

Filed:

Feb 21, 2003

Appl. No.:

10/372032

Inventors:

Courtney Coyne - Pasadena CA, US
Leanna Levine - Redondo Beach CA, US
Adrian Hightower - Pasadena CA, US
Marci Pezzuto - Altadena CA, US
Christoph Karp - Pasadena CA, US
Steven Hobbs - West Hills CA, US
Terence Flynn - Altadena CA, US

Assignee:

Nanostream, Inc.

International Classification:

G01N001/10

US Classification:

422/100000, 436/180000

Abstract:

Substantially sealed microfluidic devices for performing pipettorless ratiometric dilution are provided. In one embodiment, valves are disposed between and permit selective fluid communication between multiple chambers of a series of chambers. In another embodiment, a mixing chamber receives fluid from an inlet port and is in donative fluid communication with multiple receiving chambers each having a small volume than the mixing chamber. Active mixing means may be provided, including moveable magnetic elements, sonication, and mixing channels coupled with fluid transport means. A material transport system may be used with a non-electrokinetic pipettorless dilution system for transporting sample, diluent, and combinations thereof within the device.