Teresa Y Chan

US Patent:

7939283, May 10, 2011

Filed:

Jul 12, 2002

Appl. No.:

10/194638

Inventors:

Teresa Chan - Wayland MA, US
Joseph F. Lawlor - Arlington MA, US
Gordon C. Siek - Somerville MA, US
Arleen Chase - Billerica MA, US
Joseph D. Musto - Dover MA, US

Assignee:

Fisher Scientific Company L.L.C. - Pittsburgh PA

International Classification:

G01N 33/53
G01N 33/543

US Classification:

435 75, 435 71, 435 794, 435 792, 435 4, 436518, 436523, 436525, 436528

Abstract:

Assays, such as immunoassays, and related articles are disclosed.

US Patent:

43116906, Jan 19, 1982

Filed:

Jun 14, 1979

Appl. No.:

6/048628

Inventors:

Robert J. Buehler - Holliston MA
Teresa H. Chan - Burlington MA
Franklin Lim - Richmond VA

Assignee:

Damon Corporation - Needham Heights MA

International Classification:

G01N 3356
A61K 4300
B01J 1302

US Classification:

424 1

Abstract:

Disclosed is a process for determining the concentration of unbound hormone, e. g. , thyroxine, cortisol, or testosterone, in a liquid sample containing hormone and protein capable of binding the hormone. The sample is incubated with antibody specific to the hormone to be detected and a distinguishable analogue of the hormone, both of which are separated from the sample by semipermeable membranes capable of excluding the passage of natural protein and antibody but which allow passage of the hormone and its analogue. Preferably, the antibody and analogue are contained in semipermeable microcapsules. Free hormone in the sample permeates the membranes and competes for sites of attachment to the antibody with the analogue. The antibody and free hormones are then separated. The amount of analogue present either in association with the antibody or in the remainder of the reaction system is indicative of the level of free hormone originally present in the sample.