David Hanzel - Palo Alto CA, US Geoff Otto - Santa Clara CA, US Devon Murphy - Mountain View CA, US Paul Peluso - Hayward CA, US Thang Pham - Mountain View CA, US David Rank - Palo Alto CA, US Paul Mitsis - Trenton NJ, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
David Hanzel - Palo Alto CA, US Jonas Korlach - Menlo Park CA, US Paul Peluso - Hayward CA, US Geoffrey Otto - Santa Clara CA, US Thang Pham - Mountain View CA, US David Rank - Palo Alto CA, US Stephen Turner - Menlo Park CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12N 11/14
US Classification:
435176
Abstract:
Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.
Protein Engineering Strategies To Optimize Activity Of Surface Attached Proteins
David Hanzel - Palo Alto CA, US Jonas Korlach - Menlo Park CA, US Paul Peluso - Hayward CA, US Geoff Otto - Santa Clara CA, US Thang Pham - Mountain View CA, US David Rank - Palo Alto CA, US Stephen Turner - Menlo Park CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
Isolated and/or recombinant enzymes that include surface binding domains, surfaces with active enzymes bound to them and methods of coupling enzymes to surfaces are provided. Enzymes can include large and/or multiple surface coupling domains for surface coupling.
David Hanzel - Palo Alto CA, US Jonas Korlach - Menlo Park CA, US Paul Peluso - Hayward CA, US Geoffrey Otto - Santa Clara CA, US Thang Pham - Mountain View CA, US David Rank - Palo Alto CA, US Stephen Turner - Menlo Park CA, US
Assignee:
Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12N 9/96
US Classification:
435188
Abstract:
Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.
David K. Hanzel - Palo Alto CA, US Geoff Otto - San Carlos CA, US Devon Murphy - Mountain View CA, US Paul Peluso - San Carlos CA, US Thang Pham - Mountain View CA, US David R. Rank - Pacific Grove CA, US Paul Mitsis - Trenton NJ, US Fred Christians - Los Altos Hills CA, US Arek Bibillo - Cupertino CA, US Insil Park - Fremont CA, US Sonya Clark - Oakland CA, US John Lyle - Redwood Shores CA, US
International Classification:
C12N 9/12
US Classification:
435194
Abstract:
Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
Methods And Compositions For Single Molecule Composition Loading
The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.
Methods And Compositions For Single Molecule Composition Loading
The present invention provides methods, compositions, and systems for distributing single polymerase molecules into array regions. In particular, the methods, compositions, and systems of the present invention result in a distribution of single polymerase molecules into array regions at a percentage that is larger than the percentage expected to be occupied under a Poisson distribution.
- Menlo Park CA, US Paul S. Peluso - Newark CA, US David K. Hanzel - Palo Alto CA, US Geoff Otto - Brookline MA, US Thang Pham - Mountain View CA, US Fred Christians - Los Altos Hills CA, US Arekadiusz Bibillo - Cupertino CA, US Insil Park - Fremont CA, US Sonya Clark - Oakland CA, US John Lyle - Fremont CA, US
International Classification:
C12N 9/12 C12Q 1/6869
Abstract:
Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
Kaiser Permanente Medical GroupKaiser Permanente Medical Group Family Medicine 10850 Arrow Rte, Rancho Cucamonga, CA 91730 (909)9880379 (phone), (909)4773905 (fax)
Education:
Medical School Western Univ of Health Sciences College of Osteopathic Medicine of the Pacific Graduated: 1996
Procedures:
Destruction of Benign/Premalignant Skin Lesions Electrocardiogram (EKG or ECG) Hearing Evaluation Vaccine Administration
Dr. Pham graduated from the Western Univ of Health Sciences College of Osteopathic Medicine of the Pacific in 1996. He works in Rancho Cucamonga, CA and specializes in Family Medicine. Dr. Pham is affiliated with Kaiser Permanente Fontana Medical Center.
Dr. Pham graduated from the University of South Florida College of Medicine at Tampa in 1994. He works in Orlando, FL and specializes in Family Medicine.
Aug 2012 to 2000Ming Restaurant Portland, ME Sep 2009 to May 2010 Restaurant Operation Assistant ManagerPerfect Nail Salon Indiana, PA Aug 2008 to Aug 2009 Salon Assistant ManagerSolectron, Inc Milpitas, CA Jul 1996 to Jul 2008Everex, Inc Fremont, CA Jul 1993 to Apr 1994 Part Warehouse AssociateTag Sheet Metal San Jose, CA Mar 1991 to Jun 1992 CNC Operator
Alps Fund Services - Business Analyst (2013) Boston Financial Data Services - Mutual Fund Services Representative (2012-2013) Homestead CC - Grill Room/Banquet Server (2010-2011) Carriage Club - Zamboni Server (2010-2010) Pure Fishing (via Prologistix) - Bulk Puller (2010-2010) Victoria's Secret - Sales Associate (2009-2010)
About:
Semi-Annual Harmonica learner, Pink Martini listener, Lemon Drop drinker, Marathon/Triathlon finisher, Very occasional tweeter, Long-Distance Liverpool follower, Zombie movie watcher, Non-committed ph...
Tagline:
I drink tap water from a smartwater bottle.
Thang Pham
Work:
Renesas Design Viet Nam - SW Engineer (2)
Education:
ĐH Sư Phạm Kỹ Thuật TP HCM - IT
Thang Pham
Work:
C500 - Cong nhan (2010)
Education:
Tran khat chan - Chinhtri
Thang Pham
Work:
Truyền hình K+
Education:
Bưu Chính Viễn Thông
Thang Pham
Work:
Trên cao - Trưởng phòng mộng (1999)
About:
Thì Thắng đây, tìm đúng rồi đấy...
Tagline:
Sống trên đời sống cần có một tấm lòng, để làm gì em biết không, để gió cuốn đi, để gió cuốn đi...!
Bragging Rights:
Ví dụ: đã tốt nghiệp phổ thông, và có 3 con... ha ha
Thang Pham
Education:
University of Western Australia - Marketing
Tagline:
Finding out about Google+
Thang Pham
Work:
Trường cao đẳng giao thông vận tải - Sinh viên (2010)
Leo Carrillo Elementary School Westminster CA 1995-Present, Dr. Gustavus Brown Elementary School Waldorf MD 1996-2000, Apollo Beach Elementary School Apollo Beach FL 1997-Present