Thomas H Goodman

US Patent:

6455288, Sep 24, 2002

Filed:

Apr 8, 1993

Appl. No.:

08/044857

Inventors:

Edward Benjamin Jakobovits - Menlo Park CA
Joy L. Silen - Belmont CA
Mark J. Levy - San Jose CA
Thomas C. Goodman - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA
Robert M. Caldwell - San Carlos CA
Richard R. Bott - Burlingame CA
Christopher Charles Barnett - South San Francisco CA

Assignee:

Dade Behring Marburg GmbH - Marburg
Genencor International, Inc. - San Francisco CA

International Classification:

C12N 1552

US Classification:

435190, 4352523, 4353201, 536 232

Abstract:

The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates-to the use of conjugates of an analyte or analyte analog and a mutant NAD dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.

US Patent:

60635657, May 16, 2000

Filed:

May 26, 1995

Appl. No.:

8/451706

Inventors:

Thomas C. Goodman - Mountain View CA
Edwin F. Ullman - Atherton CA

Assignee:

Dade Behring Marburg GmbH - Marburg

International Classification:

C12P 1934
C12Q 168
G01N 3353
C07H 2104

US Classification:

435 6

Abstract:

A method for the detection of a polynucleotide target sequence is described. The method involves the formation of a covalent or non-covalent bonded pair of nucleotide sequences formed in response to a target polynucleotide sequence, adding nucleotide sequence specific binding proteins each capable of binding one member of the pair of nucleotide sequences, and detecting the specific binding proteins completed to the pair of nucleotide sequences.

US Patent:

53976983, Mar 14, 1995

Filed:

Nov 2, 1993

Appl. No.:

8/146297

Inventors:

Thomas C. Goodman - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA
Samuel Rose - Mountain View CA

Assignee:

Syntex (U.S.A.) Inc. - Palo Alto CA

International Classification:

C12Q 168
C12P 1934

US Classification:

435 6

Abstract:

A method is disclosed for producing multiple copies of a primary polynucleotide sequence located at the 3' terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template-dependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more site specific cleavage sequences, (b) cleaving into fragments said extension at cleavable polynucleotide sequences in the presence of means for specifically cleaving said cleavable polynucleotide sequences when said extension is hybridized with said site specific cleavage sequences, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection.

US Patent:

51852438, Feb 9, 1993

Filed:

Aug 25, 1988

Appl. No.:

7/236967

Inventors:

Edwin F. Ullman - Atherton CA
Thomas C. Goodman - Mountain View CA
Paul D. Stull - Mountain View CA

Assignee:

Syntex (U.S.A.) Inc. - Palo Alto CA

International Classification:

C12Q 168
C12P 1934
G01N 33566
C01H 1512

US Classification:

435 6

Abstract:

A method is disclosed for detecting the presence of a target nucleotide sequence in a polynucleotide. The method comprises hybridizing a first nucleotide sequence and a second nucleotide sequence to non-contiguous portions of a target nucleotide sequence, covalently attaching the first and second sequences when they are hybridized to the target sequence, and determining the presence of covalently attached first and second sequences. The presence of the covalently attached first and second sequences is related to the presence of the target nucleotide sequence. The invention may be applied to target nucleotide sequences in DNA or RNA. Specific target nucleotide sequences of interest will frequently be characteristic of particular microorganisms, viruses, viroids, or genetic characteristics, including genetic abnormalities.

US Patent:

61240907, Sep 26, 2000

Filed:

May 9, 1995

Appl. No.:

8/438149

Inventors:

Samuel Rose - Mountain View CA
Thomas C. Goodman - Mountain View CA
Linda M. Western - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA

Assignee:

Behringwerke AG - Marburg

International Classification:

C12Q 168
C12P 1934

US Classification:

435 6

Abstract:

A method is disclosed for determining the presence of a polynucleotide analyte in a sample suspected of containing the analyte. The method comprises (a) forming as a result of the presence of an analyte a single stranded polynucleotide comprising a target polynucleotide binding sequence flanked by first and second polynucleotide sequences that differ from the sequence of the analyte or a sequence complementary to the analyte sequence, (b) forming multiple copies of the single stranded polynucleotide, and (c) detecting the single stranded polynucleotide. Also disclosed is a method of producing at least one copy of a single stranded polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template dependent polynucleotide polymerase an extension of a polynucleotide primer at least the 3'-end of which has at least a 10 base sequence hybridizable with a second sequence flanking the 3'-end of the single stranded polynucleotide, the second sequence being partially or fully complementary with at least a 10 base first sequence flanking the 5' end of the single stranded polynucleotide, (b) dissociating the extended polynucleotide primer and the single stranded polynucleotide, (c) repeating step a and (d) dissociating the extended polynucleotide primer and the copy of the single stranded polynucleotide.

US Patent:

60935375, Jul 25, 2000

Filed:

Apr 22, 1993

Appl. No.:

8/051512

Inventors:

Thomas C. Goodman - Mountain View CA
Edwin F. Ullman - Atherton CA

Assignee:

Dade Behring Marburg GmbH - Marburg

International Classification:

C12Q 168
C12P 1934
C07K 1300

US Classification:

435 6

Abstract:

A method for the detection of a polynucleotide target sequence is described. The method involves the formation of a covalent or non-covalent bonded pair of nucleotide sequences formed in response to a target polynucleotide sequence, adding nucleotide sequence specific binding proteins each capable of binding one member of the pair of nucleotide sequences, and detecting the specific binding proteins complexed to the pair of nucleotide sequences.

US Patent:

54787297, Dec 26, 1995

Filed:

Apr 28, 1994

Appl. No.:

8/234456

Inventors:

Reuel B. Van Atta - Mountain View CA
Thomas C. Goodman - Mountain View CA
Edwin F. Ullman - Atherton CA

Assignee:

Syntex (U.S.A.) Inc. - Palo Alto CA

International Classification:

G01N 33546
G01N 33577

US Classification:

435 793

Abstract:

This invention pertains to methods to detect a compound in the presence of a homolog that is immunologically related to the analyte. The invention is particularly suited for the detection of homocysteine in the presence of cysteine. The methods of this invention involve chemically modifying both the analyte and the homolog to increase their immunogenicity and facilitate antibody recognition. More importantly, this modification is done to make these compounds immunologically distinct. Antibodies to the immunologically distinct compounds are then prepared. An assay protocol comprises chemically modifying the analyte and homolog and then immunochemically detecting the modified analyte by means of the aforementioned antibodies.

US Patent:

49943685, Feb 19, 1991

Filed:

Jul 23, 1987

Appl. No.:

7/076807

Inventors:

Thomas C. Goodman - Mountain View CA
Martin Becker - Palo Alto CA
Edwin F. Ullman - Atherton CA
Samuel Rose - Mountain View CA

Assignee:

Syntex (U.S.A.) Inc. - Palo Alto CA

International Classification:

C12Q 168
C12P 1934
G01N 33566
G01N 3348

US Classification:

435 6

Abstract:

A method is disclosed for producing multiple copies of a primary polynucleotide sequence located at the 3' terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template-dependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more site specific cleavage sequences, (b) cleaving into fragments said extension at cleavable polynucleotide sequences in the presence of means for specifically cleaving said cleavable polynucleotide sequences when said extension is hybridized with said site specific cleavage sequences, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection.