Thomas Leo Klug

US Patent:

58540091, Dec 29, 1998

Filed:

Sep 18, 1996

Appl. No.:

8/715406

Inventors:

Thomas L. Klug - West Chester PA

Assignee:

Immuna Care Corporation - Bethlehem PA

International Classification:

G01N 3353

US Classification:

435 793

Abstract:

Diagnostic/prognostic methods are provided for screening for pathologies wherein an alteration in estrogen metabolism is indicative of a pathology or a susceptibility thereto which comprise detecting and/or quantifying directly in tissues and body fluids of mammals abnormal levels of estrone metabolites and their glucuronide conjugates. Particularly preferred methods involve the use of the 16OHE1-, 2OHE1- or 2MeoE1-glucuronide fraction, i. e. , the fraction which contains the metabolite and its 3-glucuronide conjugate. Methods of preparing reagents to detect said 16OHE1-, 2OHE1-, and 2MeoE1-glucuronide fraction in tissues and body fluids are disclosed as well as test kits for performing the disclosed assays.

US Patent:

59622423, Oct 5, 1999

Filed:

Aug 22, 1997

Appl. No.:

8/917650

Inventors:

Thomas L. Klug - Tampa FL

Assignee:

Immuna Care Corporation - Bethlehem PA

International Classification:

G01N 3353
G01N 33483
G01N 3350
G01N 33532

US Classification:

435 793

Abstract:

Diagnostic/prognostic methods are provided for screening for pathologies wherein an alteration in estrogen metabolism is indicative of a pathology or a susceptibility thereto which comprise detecting and/or quantifying directly in tissues and body fluids of mammals abnormal levels of estrone metabolites and their glucuronide conjugates. Particularly preferred methods involve the use of the 16OHE1-glucuronide fraction, i. e. , the fraction which contains 16. alpha. -hydroxyestrone (16OHE1) and its conjugates, 16OHE1-3-glucuronide. Methods of preparing reagents to detect said 16OHE1-glucuronide fraction in tissues and body fluids are disclosed as well as test kits for performing the disclosed assays.