Thomas A Stamato

US Patent:

48307262, May 16, 1989

Filed:

Feb 3, 1988

Appl. No.:

7/151651

Inventors:

Thomas D. Stamato - Medford NJ
Nicholas Denko - Philadelphia PA

Assignee:

The Wistar Institute - Philadelphia PA

International Classification:

G01N 2726

US Classification:

204299R

Abstract:

A method of separating DNA molecules by gel electrophoresis is disclosed which employs alternate application of high and low strength electric fields in opposite directions to a gel matrix containing DNA. The high strength field is maintained for a shorter interval than the low strength field, and the ratio of the product of the low strength field and its pulse interval to the product of the high strength field and its pulse interval is greater than 4:1 and sufficient to produce a net migration of the DNA molecules in the direction of the low strength field.

US Patent:

20150031567, Jan 29, 2015

Filed:

Sep 20, 2011

Appl. No.:

14/345745

Inventors:

Robert C. Getts - Collegeville PA, US
Thomas D. Stamato - Medford NJ, US
Radharani Duttagupta - Foster City CA, US
Keith W. Jones - Sunnyvale CA, US

Assignee:

Genisphere, LLC - Hatfield PA

International Classification:

C12Q 1/68

US Classification:

506 9, 506 16

Abstract:

Methods for diagnosing inflammatory bowel disease and ulcerative colitis using miRNA biomarkers for these diseases are provided. Differential expression of the miRNA biomarkers in blood fractions, e.g., platelets, of diseased individuals as compared to expression levels in normal individuals indicates the presence of IBD or ulcerative colitis. Also provided are microarrays for use in the diagnostic methods, wherein the features of the microarray consist essentially of nucleic acid sequences that hybridize to the miRNA biomarkers and normalization controls.