Timothy Thomas Bell

US Patent:

20200018704, Jan 16, 2020

Filed:

Jul 11, 2018

Appl. No.:

16/033064

Inventors:

- Phoenix AZ, US
Timothy Bell - Phoenix AZ, US
Frances Ginn Howard - Phoenix AZ, US
Jonus Reyna - Tempe AZ, US

Assignee:

VISIONGATE, INC. - Phoenix AZ

International Classification:

G01N 21/64

Abstract:

A method for enhancing gating performance of a cell sorter to prepare an enriched specimen for optical tomography cell analysis includes introducing a specimen into a FACS to generate 2D event data; generating a first scatterplot of the 2D data; identifying target objects; constructing a boundary within the first scatterplot to produce a first gate; counting target objects within the first gate; comparing the number of target objects within the first gate to a first predetermined value and adjusting the first gate as necessary. A boundary around a set of target objects is constructed in a second scatterplot to produce a subset second gate and target objects within the second gate are counted and the count compared to a second predetermined value. When a boundary around target objects meets specifications the first and second gates are stored in memory and used to enrich patient specimens.

US Patent:

20170003267, Jan 5, 2017

Filed:

Jun 30, 2015

Appl. No.:

14/788015

Inventors:

- Phoenix AZ, US
Rahul Katdare - Tempe WA, US
Chris Presley - Phoenix AZ, US
Timothy Bell - Phoenix AZ, US

Assignee:

VISIONGATE, INC. - Phoenix AZ

International Classification:

G01N 33/483

Abstract:

A cytological analysis test for 3D cell classification from a specimen. The method includes isolating and preserving a cell from the specimen and enriching the cell before embedding the enriched cell into an optical medium. The embedded cell is injected into a capillary tube where pressure is applied until the cell appears in a field of view of a pseudo-projection viewing subsystem to acquire a pseudo-projection image. The capillary tube rotates about a tube axis to provide a set of pseudo-projection images for each embedded cell which are reconstructed to produce a set of 3D cell reconstructions. Reference cells are classified and enumerated and a second cell classifier detects target cells. An adequacy classifier compares the number of reference cells against a threshold value of enumerated reference cells to determine specimen adequacy.