Tracey Ann Hamilton

US Patent:

20030049719, Mar 13, 2003

Filed:

Sep 10, 2001

Appl. No.:

09/949572

Inventors:

John Petrali - Churchville MD, US
Robert Kan - Abingdon MD, US
Tracey Hamilton - Bel Air MD, US

International Classification:

G01N033/48
A01N001/02
G01N001/30

US Classification:

435/040500, 435/002000

Abstract:

A method is described of viewing a single tissue sample with a light microscope and an electron microscope. A tissue sample is cryopreserved, cryo sectioned and then floated in a cryoprotectant solution on a surface of a glass slide or in a well and is viewed by light microscopy. The step of floating the tissue sample does not destroy said tissue sample for subsequent electron microscope viewing. The same tissue sample is then prepared by conventional ultrastructural processing for viewing by electron microscopy. The data from the light microscope and electron microscope images of selected sites of the same tissue sample can be compared and analyzed.