Ulrich Y Schaff

US Patent:

20190210015, Jul 11, 2019

Filed:

Sep 14, 2017

Appl. No.:

15/704860

Inventors:

- Albuquerque NM, US
Ulrich Y. Schaff - Livermore CA, US
Gregory J. Sommer - Livermore CA, US

International Classification:

B01L 3/00
G01N 15/04
G01N 33/543

Abstract:

Embodiments of the present invention are directed toward devices, systems, and method for conducting nucleic acid purification and quantification using sedimentation. In one example, a method includes generating complexes which bind to a plurality of beads in a fluid sample, individual ones of the complexes comprising a nucleic acid molecule such as DNA or RNA and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

US Patent:

20190195865, Jun 27, 2019

Filed:

Feb 29, 2016

Appl. No.:

15/056870

Inventors:

- Albuquerque NM, US
Ulrich Y. Schaff - Livermore CA, US
Gregory Jon Sommer - Livermore CA, US

International Classification:

G01N 33/53

Abstract:

Embodiments of the present invention are directed toward devices, system and method for conducting toxin activity assay using sedimentation. The toxin activity assay may include generating complexes which bind to a plurality of beads in a fluid sample. The complexes may include a target toxin and a labeling agent, or may be generated due to presence of active target toxin and/or labeling agent designed to be incorporated into complexes responsive to the presence of target active toxin. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a lower density than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

US Patent:

20180037960, Feb 8, 2018

Filed:

Oct 19, 2017

Appl. No.:

15/788492

Inventors:

- Albuquerque NM, US
Ulrich Y. Schaff - Livermore CA, US
Gregory Jon Sommer - Maple Grove MN, US

International Classification:

C12Q 1/68

Abstract:

A system and methods for detection of a nucleic acid including forming a plurality of nucleic acid detection complexes are described, each of the complexes including a nucleic acid analyte, a detection agent and a functionalized probe. The method further including binding the nucleic acid detection complexes to a plurality of functionalized particles in a fluid sample and separating the functionalized particles having the nucleic acid detection complexes bound thereto from the fluid sample using a density media. The nucleic acid analyte is detected by detecting the detection agent.

US Patent:

20140154816, Jun 5, 2014

Filed:

Mar 16, 2012

Appl. No.:

13/423050

Inventors:

Ulrich Y. SCHAFF - Livermore CA, US
Chung-Yan Koh - Dublin CA, US
Gregory J. Sommer - Livermore CA, US

International Classification:

G01N 33/53
B82Y 15/00

US Classification:

436501, 422 69, 977774, 977902

Abstract:

Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.