Vasan K Sambandamurthy

US Patent:

7758874, Jul 20, 2010

Filed:

Apr 19, 2005

Appl. No.:

11/109056

Inventors:

Barry Bloom - Cambridge MA, US
Mary K. Hondalus - Crawford GA, US
Samantha Sampson - Brookline MA, US
Vasan Sambandamurthy - Bronx NY, US

Assignee:

Albert Einstein College of Medicine of Yeshiva University - Bronx NY
President and Fellows of Harvard College - Cambridge MA

International Classification:

A61K 39/04
A61K 49/00
A61K 39/00

US Classification:

4242481, 424 91, 424 92, 4241841, 4241851, 4242341, 4241901, 435243, 4352531, 536 231, 536 237

Abstract:

Non-naturally occurring mycobacteria in the complex are provided. These mycobacteria have a deletion of an RD1 region or a region controlling production of a vitamin, and exhibit attenuated virulence in a mammal when compared to the mycobacteria without the deletion. Also provided are non-naturally occurring mycobacteria that have a deletion of a region controlling production of lysine, and mycobacteria comprising two attenuating deletions. Vaccines comprising these mycobacteria are also provided, as are methods of protecting mammals from virulent mycobacteria using the vaccines. Also provided are methods of preparing these vaccines which include the step of deleting an RD1 region or a region controlling production of a vitamin or the amino acids leucine and lysine from a mycobacterium in the complex. Embodiments of these mycobacteria, vaccines and methods, encompassing mycobacteria comprising a leucine auxotrophy and a pantothenate auxotrophy, are also provided.

US Patent:

20040001866, Jan 1, 2004

Filed:

Jan 24, 2003

Appl. No.:

10/351452

Inventors:

William Jacobs - Pelham NY, US
Tsungda Hsu - Bronx NY, US
Stoyan Bardarov - Bronx NY, US
Vasan Sambandamurthy - Worcester MA, US

International Classification:

A61K039/04
C12N001/21

US Classification:

424/248100, 435/252300

Abstract:

Non-naturally occurring mycobacteria in the complex are provided. These mycobacteria have a deletion of an RD1 region or a region controlling production of a vitamin, and exhibit attenuated virulence in a mammal when compared to the mycobacteria without the deletion. Also provided are non-naturally occurring mycobacteria that have a deletion of a region controlling production of lysine, and mycobacteria comprising two attenuating deletions. Vaccines comprising these mycobacteria are also provided, as are methods of protecting mammals from virulent mycobacteria using the vaccines. Also provided are methods of preparing these vaccines which include the step of deleting an RD1 region or a region controlling production of a vitamin from a mycobacterium in the complex.