Victoria Ann Ploplis

US Patent:

50413791, Aug 20, 1991

Filed:

Mar 14, 1988

Appl. No.:

7/168109

Inventors:

Malcolm J. Fraser - South Bend IN
Elliot D. Rosen - South Bend IN
Victoria A. Ploplis - South Bend IN

Assignee:

American Biogenetic Science, Inc. - Copiague NY

International Classification:

C12N 701
C12N 1509
C12N 1585

US Classification:

4352351

Abstract:

The present invention relates to recombinant vector/host systems which can direct the expression of foreign genes under the control of the Heliothis polyhedrin promoter. Using the systems of the present invention, a heterologous gene of interest can be expressed as an unfused peptide or protein, a fusion protein, or as a recombinant occlusion body which comprises crystallized polyhedrin fusion proteins bearing the heterologous gene product on the surface of or within the occlusion body. The recombinant proteins or occlusion bodies of the present invention have uses in vaccine formulations and immunoassays, as biological insecticides, and as expression systems for the production of foreign peptides or proteins.

US Patent:

48700231, Sep 26, 1989

Filed:

Feb 8, 1988

Appl. No.:

7/153736

Inventors:

Malcolm J. Fraser - South Bend IN
Elliot D. Rosen - South Bend IN
Victoria A. Ploplis - South Bend IN

Assignee:

American Biogenetic Sciences, Inc. - Copiague NY

International Classification:

C12N 700
C12N 1500
C12N 100
C12P 2100

US Classification:

435320

Abstract:

The present invention is directed to recombinant baculoviruses which encode fusion polyhedrin proteins capable of forming occlusion bodies containing foreign peptides. The recombinant baculoviruses of the invention are formed by insertion into or replacement of regions of the polyhedrin gene that are not essential for occlusion body formation, with foreign DNA fragments by recombinant DNA techniques. The recombinant occlusion bodies produced in accordance with the present invention have uses in vaccine formulations, immunoassays, immobilized enzyme reactions, as biological insecticides, and as expression vectors.