Virginia D Miller

US Patent:

53388429, Aug 16, 1994

Filed:

May 22, 1992

Appl. No.:

7/890317

Inventors:

Ralph R. Isberg - Brookine MA
Virginia Miller - Los Angeles CA
Stanley Falkow - Portola Valley CA

Assignee:

The Board of Trustees of Leland Stanford Jr. University - Stanford CA

International Classification:

C12N 1531
C12N 121
C12Q 168

US Classification:

536 237

Abstract:

Novel methods and microorganisms are provided, where novel genetic mammalian cell invasive capability is imparted to a microorganism by the introduction of an exogenous inv gene. The resulting organisms are then capable of binding to mammalian cells and are transferred to the cytoplasm. Other novel genetic capabilities may be imparted to the unicellular microorganism, which may serve as a vaccine for one or more pathogens or may introduce genetic capabilities or foreign molecules into a mammalian host cell. The sequences may be used for an in vitro screen for pathogenicity.

US Patent:

53106547, May 10, 1994

Filed:

Apr 19, 1989

Appl. No.:

7/340375

Inventors:

Ralph R. Isberg - Brookline MA
Virginia Miller - Los Angeles CA
Stanley Falkow - Portola Valley CA

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Stanford CA

International Classification:

C12Q 168
C12N 1531

US Classification:

435 6

Abstract:

Novel methods and microorganisms are provided, where novel genetic mammalian cell invasive capability is imparted to a microorganism by the introduction of an exogenous inv or ail gene. The resulting organisms are then capable of binding to mammalian cells and are transferred to the cytoplasm. Other novel genetic capabilities may be imparted to the unicellular microorganism, which may serve as a vaccine for one or more pathogens or may introduce genetic capabilities or foreign molecules into a mammalian host cell. The sequences may be used for an in vitro screen for pathogenicity.