Walter F Gilbert

US Patent:

44119941, Oct 25, 1983

Filed:

Jun 8, 1978

Appl. No.:

5/913533

Inventors:

Walter Gilbert - Cambridge MA
Stephanie A. Broome - Cambridge MA
Lydia J. Villa-Komaroff - Boston MA
Argiris A. Efstratiadis - Cambridge MA

Assignee:

The President and Fellows of Harvard College - Cambridge MA

International Classification:

C12P 2104
C12P 2100
C12N 1500
C12N 100

US Classification:

435 71

Abstract:

A plasmid or phage gene for a periplasmic or extracellular bacterial protein is cleaved, a double-stranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.

US Patent:

43383971, Jul 6, 1982

Filed:

Apr 11, 1980

Appl. No.:

6/139225

Inventors:

Walter Gilbert - Cambridge MA
Karen Talmadge - Cambridge MA

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12P 2100

US Classification:

435 68

Abstract:

A method is provided for synthesizing within a bacterial host, and secreting through the membrane of the host, a selected mature protein or polypeptide. The method involves: (a) cleaving a cloning vehicle to form a cleavage site after a promoter of either (1) a bacterial or phage gene within the cloning vehicle or (2) a DNA fragment of the bacterial or phage gene; (b) forming a hybrid gene by inserting into the cleavage site a non-bacterial DNA fragment which codes for precursor of the selected protein or polypeptide, including the signal sequence of the selected protein or polypeptide; (c) transforming the host with the cloning vehicle; and then (d) culturing the transformed host to secrete the selected protein or polypeptide. By this method, mature proteins or polypeptides can be produced, free of signal sequences or other chemical substituents, such as an f-met, on the proteins or polypeptides.

US Patent:

45657858, Jan 21, 1986

Filed:

Aug 1, 1983

Appl. No.:

6/519303

Inventors:

Walter Gilbert - Cambridge MA
Stephanie A. Broome - Cambridge MA
Lydia J. Villa-Komaroff - Boston MA
Argiris A. Efstratiadis - Cambridge MA

Assignee:

The President and Fellows of Harvard College - Cambridge MA

International Classification:

C12N 100
C12N 1500
C12N 986
C07H 1512

US Classification:

435317

Abstract:

A plasmid or phage gene for a periplasmic or extracellular bacterial protein is cleaved, a double-stranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.

US Patent:

50176889, May 21, 1991

Filed:

Oct 30, 1989

Appl. No.:

7/430898

Inventors:

Walter Gilbert - Cambridge MA
Richard A. Fisher - Brookline MA
Vicki L. Sato - Cambridge MA

Assignee:

Biogen, Inc. - Cambridge MA

International Classification:

C07K 708
A61K 3702
A61K 3912

US Classification:

530326

Abstract:

This invention relates to peptides involved in the pathogenesis of human immunodeficiency virus ("HIV"). More particularly, this invention relates to peptides from the env region of the HIV genome and the use of such peptides in methods and compositions for preventing, treating, or detecting acquired immune deficiency syndrome ("AIDS") infection.

US Patent:

49436279, Jul 24, 1990

Filed:

Aug 16, 1988

Appl. No.:

7/234009

Inventors:

Walter Gilbert - Cambridge MA
Richard A. Fisher - Brookline MA
Vicki L. Sato - Cambridge MA
Johann J. Leban - Sommerville MA

Assignee:

Biogen, Inc. - Cambridge MA

International Classification:

C07K 708
C07K 710

US Classification:

530324

Abstract:

This invention relates to peptides involved in the pathogenesis of human immunodeficiency virus ("HIV"). More particularly, this invention relates to peptides from the env region of the HIV genome and the use of such peptides in methods and compositions for preventing, treating, or detecting acquired immune deficiency syndrome ("AIDS") infection.